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| Status |
Public on Jun 26, 2024 |
| Title |
Characteristics of quiescent adult neural stem cells induced by the bFGF/BMP4 combination or BMP4 alone in vitro |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bone morphogenetic protein-4 (BMP4) is involved in regulation of neural stem cells (NSCs) proliferation, differentiation, migration and survival. It was previously thought that the treatment of NSCs with BMP4 alone induces astrocytes, whereas the treatment of NSCs with the bFGF/BMP4 combination induces quiescent neural stem cells (qNSCs). In this study, we performed RNA sequencing (RNA-Seq) to compare the transcriptome profiles of BMP4-treated NSCs and bFGF/BMP4-treated NSCs, and found that both NSCs treated by these two methods were Sox2 positive qNSCs which were able to generate neurospheres. However, NSCs treated by those two methods exhibited different characteristics in state and the potential for neuronal differentiation based on transcriptome analysis and experimental results. We found that BMP4-treated NSCs tended to be in a deeper quiescent state than bFGF/BMP4-treated NSCs as the percentage of ki67-positive cells were lower in BMP4-treated NSCs. And after exposure to differentiated environment, bFGF/BMP4-treated NSCs generated more DCX-positive immature neurons and MAP2-positive neurons than BMP4-treated NSCs. Our study characterized qNSCs treated with BMP4 alone and bFGF/BMP4 combination, which laid a foundation for studying the activation mechanism of qNSCs.
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| Overall design |
NSCs were isolated from the SVZs of adult C57BL/6 mice (2 female mice and 2 male mice were used for one cell isolation experiment) aged 8-9 weeks. The cells were cultured in suspension in proliferation medium (bFGF/EGF). NSCs obtained after at least three passages were cultured in adherent in the medium with bFGF/EGF, after 16-18h, the cells were collected for RNA-seq as bFGF/EGF_0d. Then we replaced bFGF and EGF in the medium with BMP4 as BMP4-treated NSCs, and replaced EGF with BMP4 as bFGF/BMP4-treated NSCs. After 6 days, the cells were collected for RNA-seq as BMP4_6d and bFGF/BMP4_6d. The cells we used 3 biological replicas in every treatment.
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| Contributor(s) |
Xu S, Zhang X, Li Z, Liu C, Liu Q, Chai H, Yao H, Luo Y, Li S, Li C |
| Citation(s) |
38841203 |
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| Submission date |
May 09, 2024 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Sutong Xu |
| E-mail(s) |
1911450@tongji.edu.cn
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| Organization name |
Tongji university
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| Street address |
No. 500 Zhennan Road, Putuo District
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| City |
Shanghai |
| ZIP/Postal code |
200333 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1109777 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267115_genes_fpkm_expression.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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