NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE266335 Query DataSets for GSE266335
Status Public on Oct 28, 2024
Title Protective effects of red filtered light during IVF: transcriptomic analysis of murine embryos and embryo-derived EVs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Background: Light exposure of embryos during assisted reproduction affects embryo quality and implantation capacity in a wavelength dependent manner. We investigated the molecular mechanism of these light-induced changes through the comparative analysis of gene expression and regulatory miRNA profile of murine embryos cultured in dark environment and those exposed to white- or red filtered light. miRNA sequencing was used to assess the role of embryo-derived extracellular vesicles in the endometrium-embryo dialogue. Methods: In vitro cultured mouse embryos (3.5 dpc) were exposed to white or red filtered light. After 24 hours mRNA and miRNA content of the embryos as well as the miRNA content of embryo-derived extracellular vesicles were isolated and RNA-sequencing was performed. Differential expression analysis and functional enrichment analysis were used for evaluating the transcriptome results.Results: Light exposure caused transcriptomic changes in the embryos. White light upregulated apoptotic pathways, while red filtered light gave rise to the activation of regeneration pathways, including DNA repair mechanisms. Embryo-derived extracellular vesicles enclosed wavelength dependently unique miRNA cargos the target genes of which play a role in embryo implantation. In conclusion: white light upregulates apoptotic pathways, at both the transcriptome and regulatory miRNAs levels. Red filtration partially counterbalances these negative effects by shifting the cellular processes towards regeneration, including DNA repair mechanisms. Extracellular vesicles of light exposed embryos play a role in blastocyst-decidua communication through the horizontal transfer of regulatory miRNAs. Our data prove that light exposure during in vitro fertilization modifies cell function that might affect the outcome of implantation.
 
Overall design To investigate the effect of light exposure on the transcriptome of murine embryos, we have estabilished three treatment groups, embryos treated with white light, filtered red light and control.
We have sequenced the mRNA and miRNA isolated from the embryos and miRNA isolated from the embryo derived extracellular vesicles
We have performed differential expression analysis on the samples and functional enrichment to identify the significantly changed molecular pathways.
 
Contributor(s) Nagy B, Bognár Z, Csabai T, Fekete N, Buzás E, Kovács Á, Szekeres-Barthó J, Pállinger É
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 01, 2024
Last update date Oct 28, 2024
Contact name Bence Nagy
E-mail(s) nagy.bencep94@gmail.com
Phone 06 20 770 5375
Organization name Semmelweis University
Department Institute of Genetics, Cell- and Immunobiology
Lab FACS laboratory
Street address Nagyvárad tér 4
City Budapest
State/province Pest
ZIP/Postal code 1089
Country Hungary
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (28)
GSM8245161 embryo, red1, mrna
GSM8245162 embryo, red2, mrna
GSM8245163 embryo, red3, mrna
Relations
BioProject PRJNA1106783

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE266335_EV_MIRNA_FINAL.txt.gz 10.4 Kb (ftp)(http) TXT
GSE266335_emb_mirns_20231004.xlsx 82.6 Kb (ftp)(http) XLSX
GSE266335_emb_mirns_20231030.txt.gz 8.8 Kb (ftp)(http) TXT
GSE266335_mrna_results.csv.gz 308.4 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap