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Series GSE266252 Query DataSets for GSE266252
Status Public on May 17, 2024
Title Effect of depletion of PRMT2 on gene expression in the HL-60 acute myeloid leukemia cell line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Arginine methylation is catalyzed by protein arginine methyltransferases (PRMTs) and is involved in various cellular processes, including cancer development. PRMT2 expression is increased in several cancer types although its role in acute myeloid leukemia (AML) remains unknown. Here, we investigate the role of PRMT2 in a cohort of patients with AML, PRMT2 knockout AML cell lines as well as a Prmt2 knockout mouse model. In patients, low PRMT2 expressors are enriched for inflammatory signatures, including the NF-κB pathway, and show inferior survival. In keeping with a role for PRMT2 in control of inflammatory signaling, bone marrow-derived macrophages from Prmt2 KO mice display increased pro-inflammatory cytokine signaling upon LPS treatment. In PRMT2-depleted AML cell lines, aberrant inflammatory signaling has been linked to overproduction of IL6, resulting from a deregulation of the NF-κB signaling pathway, therefore leading to hyperactivation of STAT3. Together, these findings identify PRMT2 as a key regulator of inflammation in AML.
Overall design To investigate the functions of PRMT2 in AML, we used the CRISPR-Cas9 editing strategy on the HL-60 cells to generate stable PRMT2 knockout AML cell lines. We performed single-cell sorting on edited cells. Then, PRMT2 gene and protein expressions were screened on all the clones. The best KO clones (named 'AA13' and AA14') were selected and prepared for bulk RNA-sequencing, as well as the wild type (WT) HL-60 cell line as a control. All 3 samples were run on triplicates.
Contributor(s) Camille S, Romain A, Laurent D, Cyril F
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Submission date Apr 30, 2024
Last update date May 29, 2024
Contact name Laurent DELVA
Organization name Université de Bourgogne
Department Inserm U1231 (CTM)
Lab Epi2THM
Street address 7 boulevard Jeanne d’Arc
City Dijon
ZIP/Postal code 21000
Country France
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (9)
GSM8243836 HL-60 PRMT2 KO, AA13 clone, replicate 1
GSM8243837 HL-60 PRMT2 KO, AA13 clone, replicate 2
GSM8243838 HL-60 PRMT2 KO, AA13 clone, replicate 3
BioProject PRJNA1106447

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Supplementary file Size Download File type/resource
GSE266252_HL-60.KO_vs_WT.DE_analysis.tsv.gz 5.3 Mb (ftp)(http) TSV
GSE266252_RAW.tar 3.2 Mb (http)(custom) TAR (of TAB)
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Raw data are available in SRA

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