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| Status |
Public on Apr 24, 2024 |
| Title |
Stem-like T cells are associated with the pathogenesis of ulcerative colitis in humans [bulkRNAseq_MouseLPL] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the role of T cells in the pathogenesis of ulcerative colitis (UC), we analyzed colonic T cells isolated from UC patients and controls. We identified colonic CD4+ and CD8+ T lymphocyte subsets with gene expression profiles resembling stem-like progenitors, previously reported in animal models of diabetes and other contexts. Stem-like T cells were increased in inflamed areas compared to non-inflamed regions from the same patients. Furthermore, TCR sequence analysis indicated stem-like T cells were clonally related to pro-inflammatory T cells, suggesting their involvement in sustaining effectors that drive inflammation. Using an adoptive transfer colitis model, we demonstrated that CD4+ T cells deficient in BCL-6, a transcription factor promoting T cell stemness, had decreased colon T cells, reduced TH17 cells, and diminished pathogenicity. Our results establish a strong association between stem-like T cell populations and UC pathogenesis, highlighting the potential of targeting this population to improve clinical outcomes.
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| Overall design |
Splenic naïve CD4+ T cell from TcfGFP mice were isolated and transferred into Rag1 KO recipent mice to induce colitis. Then GFP+ and GFP- CD4+ T cells from the Rag1 KO mouse colon lamina propria were isolated for bulk RNA seq.
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| Contributor(s) |
Li Y, Ramírez-Suástegui C, Harris R, Castañeda-Castro FE, Giles DA, Chai J, Seumois G, Sanchez-Elsner T, Cummings F, Kronenberg M, Vijayanand P |
| Citation(s) |
38898157 |
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| Submission date |
Apr 19, 2024 |
| Last update date |
Jul 18, 2024 |
| Contact name |
Pandurangan Vijayanand |
| E-mail(s) |
vijay@lji.org
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| Organization name |
La Jolla Institute for Immunology
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| Department |
Vaccine Discovery
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| Lab |
Vijayanand Lab
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| Street address |
9420 Athena Circle
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| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| GSM8218597 |
Lamina propria, CD4 T cell, TCF7-, rep1 |
| GSM8218598 |
Lamina propria, CD4 T cell, TCF7-, rep2 |
| GSM8218599 |
Lamina propria, CD4 T cell, TCF7-, rep3 |
| GSM8218600 |
Lamina propria, CD4 T cell, TCF7+, rep1 |
| GSM8218601 |
Lamina propria, CD4 T cell, TCF7+, rep2 |
| GSM8218602 |
Lamina propria, CD4 T cell, TCF7+, rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE235665 |
Stem-like T cells are associated with the pathogenesis of ulcerative colitis in humans |
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| Relations |
| BioProject |
PRJNA1102437 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE264463_bulk_counts.txt.gz |
565.4 Kb |
(ftp)(http) |
TXT |
| GSE264463_bulk_metadata.txt.gz |
139 b |
(ftp)(http) |
TXT |
| GSE264463_bulk_tpm.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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