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Series GSE26429 Query DataSets for GSE26429
Status Public on Jun 10, 2011
Title Promotion of Direct Reprogramming by Glis1
Organism Mus musculus
Experiment type Expression profiling by array
Summary Induced pluripotent stem cells (iPSC) are generated from somatic cells by the transgene expression of three transcription factors Oct3/4, Sox2, and Klf4 (OSK), albeit at a low efficiency. The protooncogene c-Myc enhances the efficiency of iPSC generation by OSK, but it also increases the tumorigenicity of the resulting iPSC.
In the current study, we found the Gli-like transcription factor Glis1, when expressed together with OSK, to markedly enhance the generation of iPSC from both mouse and human fibroblasts. Mouse iPSC generated by OSK and Glis1 can form germline-competent chimeras. Glis1 is enriched in unfertilized oocytes and one cell-stage embryos. DNA microarray analyses revealed that Glis1 promotes multiple pro-reprogramming pathways, including Myc, Nanog, Lin28, Wnt, mesenchymal-epithelial transition (MET), and Esrrb. These results therefore demonstrated that oocyte transcription factor Glis1 effectively promote direct reprogramming during iPSC generation.
 
Overall design p53-null mouse embryonic fibroblasts were transduced with OSK and OSK+Glis1 and were used for microarray analyses.
 
Contributor(s) Maekawa M, Nakamura T, Nrita M
Citation(s) 21654807
Submission date Jan 04, 2011
Last update date Jan 12, 2017
Contact name Shinya Yamanaka
E-mail(s) yamanaka@cira.kyoto-u.ac.jp
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (4)
GSM648508 Day5 p53KO MEF OSK-1
GSM648509 Day5 p53KO MEF OSKGlis1-1
GSM648510 Day5 p53KO MEF OSK-2
This SubSeries is part of SuperSeries:
GSE26431 iPS cell generation
Relations
BioProject PRJNA142261

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26429_RAW.tar 8.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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