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| Status |
Public on Apr 17, 2024 |
| Title |
GeoMX WGS of Mtb-infected mouse lesions |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
To gain holistic of the immune landscape of lesions following infection with Mycobacterium tuberculosis, we aerosol infected C3HeB/FeJ mice with 1-3 CFU of Mtb (SA161 strain). 35 days following infection, lungs were harvested and fixed, then embedded and frozed in OCT. 10um sections were obtained, classified as necrotic or non-necrotic by visual inspection and brightfield imaging, then stained with markers delineating lesions, and subsequently processed and sampled using the nanostring GeoMX platform. We then compared the transcriptional environment of necrotic vs non-necrotic lesions
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| Overall design |
3 necrotic and 5 non-necrotric lesions from 7 mice were included. 2-4 ROIs per lesion, and one DOI from a distal area was obtain near each lesion
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| Contributor(s) |
Gern BH, Gustin AT, Gale M Jr, Gerner MY, Urdahl KB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 17, 2024 |
| Last update date |
Apr 18, 2024 |
| Contact name |
Benjamin Henry Gern |
| E-mail(s) |
benjamin.gern@seattlechildrens.org
|
| Phone |
2068843161
|
| Organization name |
Seattle Children's Research Institute
|
| Department |
Center for Global Infectious Diseases Research
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| Lab |
Gern
|
| Street address |
307 Westlake Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA1101569 |
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