GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE263427 Query DataSets for GSE263427
Status Public on May 10, 2024
Title Deciphering age-related transcriptomic changes in mouse retinal pigment epithelium
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Aging of the retinal pigment epithelium (RPE) leads to a gradual decline in RPE homeostasis over time, significantly impacting retinal health. Understanding the mechanisms underlying RPE aging is crucial for elucidating the background in which many age-related retinal pathologies develop. In this study, we compared the transcriptomes of young and aged mouse RPE and found a marked upregulation of immunogenic and proinflammatory genes in aging RPE. Additionally, aging RPE exhibited dysregulation of pathways associated with visual perception and extracellular matrix production. Research on aging in post-natal quiescent RPE is hindered by the absence of relevant in vitro models. We evaluated an in vitro model of chronologically aged primary human RPE to address this gap, which displayed gene expression patterns comparable to native-aged RPE. Profiling this in vitro aging model highlighted its potential utility in investigating cellular and molecular mechanisms of RPE aging and in preliminary testing of therapeutic compounds. Overall, our findings suggest chronological aging of RPE leads to the acquisition of a proinflammatory phenotype, impacting RPE function and serving as a significant factor in the development of age-related retinal pathologies.
Overall design We performed transcriptome profiling of ribosomal RNA depleted sequencing data from RPE/choroid of young (2-3 months) and aged (20-24 months) mice (n=4 per group).
Contributor(s) Dubey SK, Dubey R, Jung Ks, Kleinman ME
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 08, 2024
Last update date May 10, 2024
Contact name Mark Ellsworth Kleinman
Phone 423-439-5969
Organization name East Tennessee State University
Department Department of Surgery
Lab Ocular Biology and Imaging Lab
Street address Quillen College of Medicine
City Johnson City
State/province Tennessee
ZIP/Postal code 37614
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM8191508 C57BL/6J, Young mouse1, RPE/choroid, 2-3 months
GSM8191509 C57BL/6J, Young mouse2, RPE/choroid, 2-3 months
GSM8191510 C57BL/6J, Young mouse3, RPE/choroid, 2-3 months
BioProject PRJNA1097520

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE263427_RPEA_vs_RPEY_tpm.txt.gz 1.2 Mb (ftp)(http) TXT
GSE263427_RPEY_vs_RPEA_stats.diff.xlsx 1.7 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap