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Series GSE26315 Query DataSets for GSE26315
Status Public on Jun 02, 2011
Title Expression data from human amnion mesenchymal cells treated with interleukin-1-beta for 1hr and 8hr
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Premature birth continues to be a challenging pregnancy complication, and a body of literature indicates that inflammation can contribute to premature delivery by converting a receptive uterine environment to a hostile one. Cytokines have been demonstrated to provoke up-regulation of inflammatory genes (e.g. interleukin-1, 6, and 8, tumor necrosis factor-alpha, cyclooxygenase-2, and microsomal prostaglandin E synthase-1).
Using monolayer cultures of human amnion mesenchymal cells (AMCs) as a model, we aimed to detail the global programme of gene expression that occurs in response to cytokine challenge.
Overall design Human amnion mesenchymal cells were challenged with 10 ng/ml of interleukin-1-beta for 1 hour (1h) or 8 hours (8h) in serum-free media, while control cells were treated with serum free medium only (veh). The cells (veh, 1h and 8h) were subjected to RNA extraction, quantification, and evaluation, followed by hybridization on Affymetrix GeneChip® Human Genome U133A 2.0 Arrays (Platform GPL571). Experiments were repeated three times for a total of nine samples used for microarray analysis. Three biological replicates were used to ensure that any results were not biased by day-to-day variation in RNA extraction.
Contributor(s) Li R, Ackerman IV WE, Summerfield TL, Yu L, Galati P, Zhang J, Huang K, Romero R, Kniss DA
Citation(s) 21655103
Submission date Dec 27, 2010
Last update date Dec 06, 2018
Contact name Douglas A. Kniss
Organization name The Ohio State University
Department OB/GYN
Lab Laboratory of Perinatal Research
Street address 395 W. 12th Avenue
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (9)
GSM646173 amnion mesenchymal cells, no treatment, biological rep1
GSM646174 amnion mesenchymal cells, no treatment, biological rep2
GSM646175 amnion mesenchymal cells, no treatment, biological rep3
BioProject PRJNA136761

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Supplementary file Size Download File type/resource
GSE26315_RAW.tar 17.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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