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Series GSE26294 Query DataSets for GSE26294
Status Public on Dec 21, 2015
Title Functional abnormalities and changes in gene expression in fibroblasts and macrophages from the bone marrow of patients with acute myeloid leukemia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In leukemias and other malignancies of the bone marrow, little is known about the fate of fibroblasts and resident macrophages after normal hematopoietic cells are replaced by neoplastic cells. In the present investigation we used two-stage long-term bone marrow cultures to detect functional stromal cell abnormalities in acute myeloid leukemia, myelodysplastic syndromes and multiple myeloma. While fibroblasts from multiple myeloma and macrophages from multiple myeloma and myelodysplastic syndromes were functionally indistinguishable from the respective cell types from normal bone marrow, fibroblasts from patients with acute myeloid leukemia or myelodysplastic syndromes possessed a significantly lower ability to support hematopoiesis originating from co-cultured normal CD34-positive cells than fibroblasts from healthy marrow. Conversely, macrophages from acute myeloid leukemia marrow significantly enhanced the production of blood cells compared with control macrophages. Aberrant function in fibroblasts and macrophages was associated with consistent changes in the expression of genes whose products are involved in hematopoietic stem cell control, such as cytokines and regulators of the Wnt and Notch signalling pathways.
Overall design Analysis of gene expression in fibroblast and macrophage populations isolated from primary long-term bone marrow cultures (LTBMC). Adherent layers of LTBMCs were trypsinized, fibroblasts and macrophages were purified using anti-Fibroblast or CD14 MicroBeads (Miltenyi Biotec, Begisch-Gladbach, Germany), and total RNA was extracted by using the RNeasy kit from Qiagen (Hilden, Germany), as specified by the manufacturer. Global gene expression was analyzed in 4 samples each of fibroblasts and macrophages from AML patients and control subjects.
Contributor(s) Li Y, Klein-Hitpass L, Dührsen U, Jan D
Citation(s) 26121956
Submission date Dec 23, 2010
Last update date Dec 06, 2018
Contact name Ludger Klein-Hitpass
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (16)
GSM645644 A1_F_Fibroblasts_AML-Patient_1
GSM645645 A2_F_Fibroblasts_AML-Patient_2
GSM645646 A3_F_Fiboblasts_AML-Patient_3
BioProject PRJNA135055

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26294_RAW.tar 33.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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