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Series GSE262926 Query DataSets for GSE262926
Status Public on Apr 04, 2024
Title Micro bulk sequencing on murine Bleomycin-induced fibrotic lungs sorted on SA-β-Gal and GD3
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Aging is marked by a progressive decline in physiological function, leading to an increased susceptibility to age-related diseases and cancer. On the cellular level, aging correlates with an accumulation of senescent cells, which play a dual role: they are critical for tissue homeostasis, yet their over-accumulation may drive pathological aging. We recently revealed that senescent cells could overexpress immunosuppressive molecules like the ganglioside GD3 at their cell surface to evade from immune surveillance and to facilitate their tolerogenicity. We investigate which cells expressed GD3 and whether this ganglioside could be considered as a new marker associated to senescence in a model of senescence associated disease: Bleomycin-induced lung fibrosis.
 
Overall design To characterize GD3 positive cells we induced lung fibrosis in young C57Bl6/J male mice by Bleomycin instillation. After 27 days, we collected the lungs, dissociated them and labeled them for GD3 as well as a know senescence marker: SA-β-Gal. We then sorted the different fractions.
 
Contributor(s) Moskalevska I, Cherfils-Vicini J, Moudombi L, Michallet MC, Ardin M, Ferrari A
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Submission date Apr 01, 2024
Last update date Apr 04, 2024
Contact name Julien Cherfils-Vicini
E-mail(s) julien.cherfils@univ-cotedazur.fr
Organization name IRCAN - CNRS - INSERM
Street address 28 Avenue de Valombrose
City Nice
ZIP/Postal code 06107
Country France
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (15)
GSM8181568 GD3+ Bgal+ 1
GSM8181569 GD3+ Bgal- 1
GSM8181570 GD3- Bgal+ 1
Relations
BioProject PRJNA1094922

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE262926_counts.txt.gz 1.0 Mb (ftp)(http) TXT
GSE262926_tpm.txt.gz 1.6 Mb (ftp)(http) TXT
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Raw data are available in SRA

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