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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 11, 2024 |
| Title |
Stroma-derived Dickkopf-1 suppresses NK cell cytotoxicity in breast cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Effects of DKK1 on tumor cells and NK cells
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| Overall design |
Tumor cells: GFP-H2B-mApple-Thy1.1+ PyMT-BO1 cells from orthotopic breast tumors 15 days after tumor inoculation in IgG or mDKN01-treated WT mice were sorted, after exclusion of Ter119+ erythrocytes and CD45+ immune cells and extracted RNA for sequencing. NK cells: NK cells sorted from the spleen of poly I:C treated C57BL/6 mice using negative selection beads and stimulated ex vivo with rDKK1 for 4 hours. Then, further sorted cells based on CD45+CD3-NK1.1+ markers and extracted RNA for sequencing
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| Contributor(s) |
Lee S, Faccio R |
| Citation(s) |
38659818 |
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https://doi.org/10.21203/rs.3.rs-4202878/v1
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| Submission date |
Mar 28, 2024 |
| Last update date |
May 10, 2024 |
| Contact name |
Seunghyun Lee |
| E-mail(s) |
seunghyun.lee@wustl.edu
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| Phone |
3146855902
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| Organization name |
Washington University in St.Louis
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| Street address |
660 S. Euclid Ave., Box 8228
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| City |
SAINT LOUIS |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM8174652 |
PyMT tumor cells, mDKN01, 1 |
| GSM8174653 |
PyMT tumor cells, mDKN01, 2 |
| GSM8174654 |
PyMT tumor cells, mDKN01, 3 |
| GSM8174655 |
NK cells, PBS, 1 |
| GSM8174656 |
NK cells, PBS, 2 |
| GSM8174657 |
NK cells, PBS, 3 |
| GSM8174658 |
NK cells, rDKK1, 1 |
| GSM8174659 |
NK cells, rDKK1, 2 |
| GSM8174660 |
NK cells, rDKK1, 3 |
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| Relations |
| BioProject |
PRJNA1093077 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262733_RAW.tar |
19.3 Mb |
(http)(custom) |
TAR (of SF, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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