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Series GSE262466 Query DataSets for GSE262466
Status Public on May 08, 2024
Title Alveolar Macrophage Phenotypes in Schistosoma-Induced Pulmonary Hypertension
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Schistosomiasis, a prevalent cause of pulmonary hypertension (PH) globally, triggers type 2 inflammation. The role of alveolar macrophages (AMs) are not well defined. This study provides insights into the complex interplay of AMs subpopulations in Schistosoma-induced PH, shedding light on potential therapeutic targets for this global health concern.
Overall design Cx3cr1+/GFP reporter mice were intraperitoneally sensitized and then intravenously challenged with S. mansoni eggs .Lungs alveolar macrophages (AMs) were sorted from unexposed and at day 1, 3 and 7 following Schistosoma IV challenge by flow cytometry. At each time point, IMs were sorted from of a pair of age- and sex-matched mice (1 male and 1 female) and were combined for subsequent analyses. Single cells were captured using a Chromium Box (10X Genomics, Pleasanton, CA). Libraries were sequenced using the Illumina NovaSEQ 6000 sequencer (Illumina, Inc., San Diego, CA). Subsequently, RNA sequence reads were aligned to the Mus musculus GRCm39 reference genome using Cell Ranger v6 (10x Genomics). Downstream analysis was performed using the Seurat package in R.
Contributor(s) Kumar R, Kumar S, Mickael C, Fonseca Balladares D, Nolan K, Lee M, Sanders L, Tuder RM, Stenmark KR, Graham BB
Citation(s) 38779688
Submission date Mar 26, 2024
Last update date Jun 05, 2024
Contact name Rahul Kumar
Organization name University of California, San Francisco, USA
Department Medicine
Lab Graham Lab
Street address 1001 Potrero Ave, Rm.5K1
City San Francisco
State/province CA
ZIP/Postal code 94110
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM8170132 NMX_AM, scRNAseq
GSM8170133 1DIP_IV_AM, scRNAseq
GSM8170134 3D_IP_IV_AM, scRNAseq
BioProject PRJNA1091933

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE262466_RAW.tar 93.1 Mb (http)(custom) TAR (of TAR)
GSE262466_all_marker_schisto_am.csv.gz 165.3 Kb (ftp)(http) CSV
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Raw data are available in SRA

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