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Series GSE262352 Query DataSets for GSE262352
Status Public on May 29, 2024
Title Spatial transcriptome analysis of muscle sections from Icos-/- NOD mice with established myositis
Organism Mus musculus
Experiment type Other
Summary Icos-/- NOD mice are a model of spontaneous myositis. By using Nanostring GeoMx technology (Mouse WTA), we aimed to investigate the differences in gene expression between myofibers of Icos+/+ NOD (WT) mice and myofibers from Icos-/- NOD mice either in direct contact or not with large infiltrate clusters.
 
Overall design Using Nanostring technology, the transcriptomes of myofibers vs. immune cells were analysed separately by using desmin and CD45 markers. The present study includes the following AOIs: 4 AOIs from Icos+/+ NOD muscle (n=4 mice, 1 AOI/mouse, desmin+CD45-) , 6 AOIs from Icos-/- NOD muscle not directly adjacent to immune cell infiltrates (PROX, n=6 mice, 1 AOI/mouse, desmin+CD45-), 8 AOIs from Icos-/- NOD muscle directly adjacent to immune cell infiltrates (ADJ, n=6 mice, 1-2 AOI/mouse, desmin+CD45-), 8 AOIs from Icos-/- NOD muscle coresponsing to immune cell infiltrates (INF, n=6 mice, 1-2 AOI/mouse, desmin-CD45+).
 
Contributor(s) Abad C, Boyer O
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 25, 2024
Last update date May 30, 2024
Contact name Catalina Abad
E-mail(s) catalina.abad@inserm.fr
Organization name Inserm U1234
Street address 22 Blvd Gambetta
City Rouen
ZIP/Postal code 76000
Country France
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (26)
GSM8163707 Muscle, Icos-/- NOD, CD45+, INF [1_3|001|CD45+]
GSM8163708 Muscle, Icos-/- NOD, CD45-, ADJ [1_3|001|CD45-]
GSM8163709 Muscle, Icos-/- NOD, CD45-, PROX [1_3|002|CD45-]
Relations
BioProject PRJNA1091565

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Supplementary file Size Download File type/resource
GSE262352_Mm_R_NGS_WTA_v1.0.pkc.gz 1.8 Mb (ftp)(http) PKC
GSE262352_Q3_normalized_Data_WTA.xlsx 1.6 Mb (ftp)(http) XLSX
GSE262352_RAW.tar 1.6 Mb (http)(custom) TAR (of DCC)
SRA Run SelectorHelp
Raw data are available in SRA

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