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Series GSE262086 Query DataSets for GSE262086
Status Public on Mar 27, 2024
Title Human-Specific Elimination of Epithelial Siglec-XII Suppresses the Risk of CRC Initiation and Progression [baseline]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Human-specific changes in specific Siglecs is one of the reasons put forth as molecular mechanisms that could explain human proneness to developing cancers. The SIGLEC12 gene, which encodes the Siglec-XII protein mainly found on epithelial cells, has a fixed homozygous missense mutation in a critical arginine renders unable to recognize its natural ligand. Additionally, the gene harbors a polymorphic frameshift mutation that eliminates expression of the full-length protein in most humans. We hypothesized that dysfunctional Siglec-XII is involved in cancer progression in humans' epithelia. Here we report that the transgenic conditional expression of human Siglec-XII in mouse intestinal epithelia did not by itself cause carcinomas and RNA sequencing of the colons at baseline derived a gene signature without differentially expressed genes (DEGs) between controls (Villin1-Cre-ER(T2)) and Siglec-XII-expressing mice
Overall design To investigate the impact of the expression of human SIGLEC12 in mouse epithelia, we developed a knock-in mouse model that allows its conditional expression only in the villi and crypts of the small and large intestine (SIGLEC12-Villin1-Cre-ERT). SIGLEC12 knock in mice (SIGLEC12-Villin1-Cre-ERT) and controls (Villin1-Cre-ERT) received 5 days treatment (10 mg/ml) with tamoxifen, to induce SIGLEC12 expression. After 7 days of the last tamoxifen administration mice were sacrificed, colons were harvested. We then performed gene expression profiling analysis using data obtained from RNA-seq of three mice for each condition.
Contributor(s) Varki A, Ghosh P
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Submission date Mar 20, 2024
Last update date Mar 27, 2024
Contact name Pradipta Ghosh
Organization name University of California San Diego
Department Departments of Medicine and Cellular and Molecular Medicine
Street address 9500 Gilman Drive (MC 0651)
City La Jolla, CA 92093
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM8157290 Colon, Control_1_S57
GSM8157291 Colon, Control_2_S58
GSM8157292 Colon, Control_3_S59
This SubSeries is part of SuperSeries:
GSE262088 Human-Specific Elimination of Epithelial Siglec-XII Suppresses the Risk of CRC Initiation and Progression
BioProject PRJNA1090163

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Supplementary file Size Download File type/resource
GSE262086_pg-av2-expr.txt.gz 2.2 Mb (ftp)(http) TXT
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