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Series GSE262085 Query DataSets for GSE262085
Status Public on Mar 27, 2024
Title Human-Specific Elimination of Epithelial Siglec-XII Suppresses the Risk of CRC Initiation and Progression [CACO]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human-specific changes in specific Siglecs is one of the reasons put forth as molecular mechanisms that could explain human proneness to developing cancers. The SIGLEC12 gene, which encodes the Siglec-XII protein mainly found on epithelial cells, has a fixed homozygous missense mutation in a critical arginine renders unable to recognize its natural ligand. Additionally, the gene harbors a polymorphic frameshift mutation that eliminates expression of the full-length protein in most humans. We hypothesized that dysfunctional Siglec-XII is involved in cancer progression in humans' epithelia. Here we report that the forced expression of human Siglec-XII in caco-2 cells showed that differentially expressed genes in Siglec-XII cells were enriched for diverse bioenergetic processes.
 
Overall design To explore the significance of human Siglec-XII in the progression of CRCs, we used Siglec-XII non-expressing Caco-2 cell line. Cells were transfected with either pCDNA-3.1-SIGLEC12 or with empty vector (control), forcing them to exogenously express full-length Siglec-XII and stable clones were selected. We then performed gene expression profiling analysis using data obtained from RNA-seq of three cultures for each condition.
 
Contributor(s) Varki A, Ghosh P
Citation(s) 38990656
Submission date Mar 20, 2024
Last update date Sep 27, 2024
Contact name Pradipta Ghosh
E-mail(s) prghosh@ucsd.edu
Organization name University of California San Diego
Department Departments of Medicine and Cellular and Molecular Medicine
Street address 9500 Gilman Drive (MC 0651)
City La Jolla, CA 92093
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM8157282 Caco-2_pCDNA_3_1-Empty_Vector_1_S73
GSM8157283 Caco-2_pCDNA_3_1-Empty_Vector_2_S74
GSM8157284 Caco-2_pCDNA_3_1-Empty_Vector_3_S75
This SubSeries is part of SuperSeries:
GSE262088 Human-Specific Elimination of Epithelial Siglec-XII Suppresses the Risk of CRC Initiation and Progression
Relations
BioProject PRJNA1090164

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE262085_pg-avcaco-expr.txt.gz 2.7 Mb (ftp)(http) TXT
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Raw data are available in SRA

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