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Status |
Public on Mar 27, 2024 |
Title |
Human-Specific Elimination of Epithelial Siglec-XII Suppresses the Risk of CRC Initiation and Progression [CACO] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Human-specific changes in specific Siglecs is one of the reasons put forth as molecular mechanisms that could explain human proneness to developing cancers. The SIGLEC12 gene, which encodes the Siglec-XII protein mainly found on epithelial cells, has a fixed homozygous missense mutation in a critical arginine renders unable to recognize its natural ligand. Additionally, the gene harbors a polymorphic frameshift mutation that eliminates expression of the full-length protein in most humans. We hypothesized that dysfunctional Siglec-XII is involved in cancer progression in humans' epithelia. Here we report that the forced expression of human Siglec-XII in caco-2 cells showed that differentially expressed genes in Siglec-XII cells were enriched for diverse bioenergetic processes.
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Overall design |
To explore the significance of human Siglec-XII in the progression of CRCs, we used Siglec-XII non-expressing Caco-2 cell line. Cells were transfected with either pCDNA-3.1-SIGLEC12 or with empty vector (control), forcing them to exogenously express full-length Siglec-XII and stable clones were selected. We then performed gene expression profiling analysis using data obtained from RNA-seq of three cultures for each condition.
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Contributor(s) |
Varki A, Ghosh P |
Citation(s) |
38990656 |
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Submission date |
Mar 20, 2024 |
Last update date |
Sep 27, 2024 |
Contact name |
Pradipta Ghosh |
E-mail(s) |
prghosh@ucsd.edu
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Organization name |
University of California San Diego
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Department |
Departments of Medicine and Cellular and Molecular Medicine
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Street address |
9500 Gilman Drive (MC 0651)
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City |
La Jolla, CA 92093 |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE262088 |
Human-Specific Elimination of Epithelial Siglec-XII Suppresses the Risk of CRC Initiation and Progression |
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Relations |
BioProject |
PRJNA1090164 |