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Series GSE261340 Query DataSets for GSE261340
Status Public on Mar 12, 2024
Title Soma-to-Germline miRNA inheritance via Yolk Promotes Stress Resilience in Progeny [small RNA]
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary At the onset of reproduction, oviparous animals synthesize large amounts of yolk in somatic tissues to provide lipids and other nutrients to their progeny. However, whether the yolk transports other types of molecules, such as RNAs with gene regulatory functions, remains largely unexplored. Here, we have biochemically purified the yolk in the nematode Caenorhabditis elegans and show it contains microRNAs (miRNAs). We provide evidence that the yolk transports such miRNAs from the mother’s intestine to the embryos via the lipoprotein yolk receptor RME-2. These yolk-enriched miRNAs inherited by the embryos regulate the transcriptomes of developing larvae. Moreover, environmental stresses and maternal age modulate the transfer of yolk-enriched miRNAs, providing stress resilience benefits to progeny. This discovery establishes a novel paradigm in intergenerational gene regulation, where the gut-germline axis orchestrates the transmission of environmental cues through yolk-enriched miRNAs. Our work reveals a new mechanism underlying the soma-to-germline transfer of epigenetic information in animals.
 
Overall design small RNA sequencing profiles of sorted yolk by VIT-2::GFP and 2-cell embryos under different conditions of growth and genotypes.
 
Contributor(s) Aupérin N, Singh M, Bourdon L, Commere P, Chervova A, Lopez-Maestre H, Gkaraveli A, Cecere G
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Submission date Mar 11, 2024
Last update date Jul 09, 2024
Contact name Germano Cecere
E-mail(s) germano.cecere@pasteur.fr
Phone 0033140613225
Organization name Institut Pasteur
Department Development and stem cell biology
Lab Mechanisms of Epigenetic Inheritance
Street address Institut Pasteur, 28 Rue Du Docteur Roux, Batiment Monod, 4eme Etage
City Paris
ZIP/Postal code 75724
Country France
 
Platforms (1)
GPL32326 NextSeq 2000 (Caenorhabditis elegans)
Samples (43)
GSM8140769 GFP_sorted_OP50_biol_rep1
GSM8140770 GFP_sorted_OP50_biol_rep2
GSM8140771 GFP_sorted_OP50_biol_rep3
Relations
BioProject PRJNA1086516

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Supplementary file Size Download File type/resource
GSE261340_RAW.tar 82.9 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA

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