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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 17, 2024 |
| Title |
Germinal Center Dark Zone harbors ATR-dependent determinants of T-cell exclusion that are also identified in aggressive lymphoma – Visium_FFPE_Spatial_Trasciptomics_project |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The germinal center (GC) dark zone (DZ) and light zone (LZ) regions spatially separate expansion and diversification from selection of antigen-specific B-cells to ensure antibody affinity maturation and B cell memory. How GC polarity is established is poorly understood. This study provides novel insights into signals controlling asymmetric T-cell distribution between DZ and LZ regions. We identify spatially-resolved DNA damage response and chromatin compaction molecular features precluding DZ T-cell infiltration. The DZ spatial transcriptional signature linked to T-cell immune evasion clustered aggressive Diffuse Large B-cell Lymphomas (DLBCL) for differential T cell infiltration. We reveal the dependence of the DZ transcriptional core signature on the ATR kinase and dissect its role in restraining inflammatory responses contributing to estabslish an immune-repulsive imprint in DLBCL. These insights may guide ATR-focused treatment strategies bolstering immunotherapy in tumors marked by DZ transcriptional and chromatin-associated features.
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| Overall design |
Whole-genome transcriptomic profiling was performed on formalin-fixed tissue sections of mouse FFPE mesenteric lymph nodes from two samples: one with a knocked Aicda gene (B6.129P2-Aicdatm1(cre)Mnz/J, Jackson, RRID no 007770) and the other under wild-type conditions for Aicda gene (B6N;129-Ilktm1Star/J, Jackson, RRID no 023310).
grant ID : *22145*
grant title: *Linking genetic evolution, stromal and immune contextures to
unravel tumor heterogeneity in aggressive B-cell lymphomas*
name of the funding source:* A.I.R.C. (Associazione Italiana Ricerca
Cancro) *
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| Contributor(s) |
Cancila V, Morello G, Bertolazzi G |
| Citation(s) |
38562878 |
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| Submission date |
Mar 06, 2024 |
| Last update date |
Apr 18, 2024 |
| Contact name |
Claudio Tripodo |
| E-mail(s) |
lab.tripodo@gmail.com
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| Organization name |
Univerisity of Palermo
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| Department |
PROMISE
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| Lab |
Tumor immunology Unit
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| Street address |
Corso Tukory 211
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| City |
Palermo |
| ZIP/Postal code |
90134 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1084805 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE260998_RAW.tar |
425.5 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TIFF, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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