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Series GSE26059 Query DataSets for GSE26059
Status Public on Dec 15, 2010
Title Epidermal differentiation: basal vs. suprabasal keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In epidermal differentiation basal keratinocytes detach from the basement membrane, stop proliferating, and express a new set of structural proteins and enzymes, which results in an impermeable protein/lipid barrier that protects us. To define the transcriptional changes essential for this process, we purified large quantities of basal and suprabasal cells from human epidermis, using the expression of b4 integrin as the discriminating factor. The expected expression differences in cytoskeletal, cell cycle and adhesion genes confirmed the effective separation of the cell populations. Using DNA microarray chips, we comprehensively identify the differences in genes expressed in basal and differentiating layers of the epidermis, including the ECM components produced by the basal cells, the proteases in both the basal and suprabasal cells, and the lipid and steroid metabolism enzymes in suprabasal cells responsible for the permeability barrier. We identified the signaling pathways specific for the two populations, and found two previously unknown paracrine and one juxtacrine signaling pathway operating between the basal and suprabasal cells. Furthermore, using specific expression signatures, we identified a new set of late differentiation markers and mapped their chromosomal loci, as well as a new set of melanocyte-specific markers. The data represent a quantum jump in understanding the mechanisms of epidermal differentiation. Basal keratinocytes are defined as integrin b4-positive. Suprabasal sample contains all melanocytes as well.
Overall design Basal (integrin b4+), suprabasal (b4-) and total epidermal keratinocytes. In duplicate, from 2 independent donors, breast reduction surgery.
Contributor(s) Blumenberg M, Gazel A, Radoja N, Banno T, Yano S
Citation(s) 16822832
Submission date Dec 14, 2010
Last update date Dec 06, 2018
Contact name Miroslav Blumenberg
Phone 212 263-5924
Organization name NYU School of Medicine
Department Dermatology
Lab Blumenberg
Street address 455 First Ave #874
City New York
State/province NY
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (7)
GSM639879 Total epidermal cells, all layers Sample 1
GSM639880 Basal epidermal cells, Sample 1
GSM639881 Suprabasal epidermal cells, Sample 1
BioProject PRJNA135569

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26059_RAW.tar 16.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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