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Series GSE260582 Query DataSets for GSE260582
Status Public on Apr 12, 2024
Title Mitochondrial ACSS1-K-635 acetylation mimic knock-in mice exhibit altered lipid metabolism, hepatic cell senescence, and acute non-alcoholic fatty liver [2ndSet_analysis]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We generated an ACSS1-acetylation (Ac) mimic mouse, where lysine 635 was mutated to glutamine (K635Q). Male Acss1K635Q/K635Q mice were smaller with higher metabolic rate and blood acetate, and decreased liver/serum ATP and lactate levels. After a 48-hour fast, Acss1K635Q/K635Q mice presented hypothermia and liver aberrations, including enlargement, discoloration, lipid droplet accumulation, and microsteatosis – consistent with nonalcoholic fatty liver disease (NAFLD). RNAseq analysis suggested dysregulation of fatty acid metabolism, cellular senescence, and hepatic steatosis networks, consistent with NAFLD
 
Overall design Fasted and fed C57BL/6J WT and ACSS1K635Q mice were studied in the context of non-alcoholic fatty liver disease. The liver tissue was collected and prepared for RNAseq as well as for Western blotting and qPCR analysis.
 
Contributor(s) Gius D, Xu G, Schell J, Horikoshi N, Chen Y, Lai Z
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Submission date Feb 29, 2024
Last update date Apr 13, 2024
Contact name Yidong Chen
E-mail(s) cheny8@uthscsa.edu
Phone 2105629163
Organization name UT Health Science Center at San Antonio
Department Population Health Sciences
Street address 8403 Floyd Curl Drive, MSC 7784
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM8119705 liver, ACSS1K635Q, fasted, biol rep 1
GSM8119706 liver, ACSS1K635Q, control diet, biol rep 1
GSM8119707 liver, ACSS1K635Q, control diet, biol rep 2
Relations
BioProject PRJNA1082272

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE260582_RAW.tar 5.0 Mb (http)(custom) TAR (of RESULTS)
SRA Run SelectorHelp
Raw data are available in SRA

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