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Series GSE259405 Query DataSets for GSE259405
Status Public on Jan 13, 2025
Title Repetitive transcranial magnetic stimulation induces cortical layer, brain region, and protocol dependent neural plasticity [Spatial transcriptomics]
Organism Mus musculus
Experiment type Other
Summary Repetitive transcranial magentic stimulation (rTMS) is a non-invasive toll commonly used to study neural plasticity processes and treat neurological disorders. Despite the popularity of rTMS, it is still unclear what neural plasticity mechanisms are induced in the brain regions at and beyond the stimulation site, and how this varies with different rTMS protocols. Here we used spatial transcriptomics to map the neural plasticity mechanisms induced across cortical and sub-cortical regions following intermittent or continuous theta-burst stimulation protocols to the mouse sensorimotor cortex. Our results revealed that rTMS alters the expression of genes related to several cellular processes and neural plasticity mechanisms across the brain which was both brain region and rTMS protocol dependent. In the cortex, the effect of rTMS was not only dependent on the cortical region, but also the cortical layer within each cortical region. These findings uncover the neural plasticity mechanisms induced across the brain following rTMS and help inform how different stimulation protocols can be used to drive specific neural plasticity mechanisms.
 
Overall design Wild-type male C57BL/6J mice were treated with a single session of intermittent theta-burst (iTBS), continuous theta-burst (cTBS), or sham stimulation, with four mice used for each stimulatio group. Animals were euthanised three hours post-stimulation and the brains were removed and dissected along the midline. Coronal sections from the right brain hemisphere, taken approximately 0.26 - 0.74 mm anterior from bregma, were analysed using spatial transcriptomics.
 
Contributor(s) Ong RC, Tang AD
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Submission date Feb 27, 2024
Last update date Jan 14, 2025
Contact name Alexander Tang
E-mail(s) alex.tang@uwa.edu.au
Phone (08) 6488 2245
Organization name University of Western Australia
Department School of Biomedical Science
Lab Healthy Brain Ageing and Repair Lab
Street address 35 Stirling Highway, University of Western Australia
City Perth
State/province Western Australia
ZIP/Postal code 6009
Country Australia
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM8115651 Brain, sham, biol rep 1
GSM8115652 Brain, sham, biol rep 2
GSM8115653 Brain, sham, biol rep 3
Relations
BioProject PRJNA1081430

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Supplementary file Size Download File type/resource
GSE259405_RAW.tar 560.6 Mb (http)(custom) TAR (of CSV, H5, JPG, JSON, MTX, PNG, TSV)
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Raw data are available in SRA

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