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| Status |
Public on Mar 01, 2024 |
| Title |
Cas13bt3 knockdown of VEGFA mRNA in mammalian cells and mouse retina [RNA-Seq] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: We report expression of a new Cas13 enzyme in retinal cells and evaluate its efficacy in targeting the VEGFA mRNA to establish safety and efficacy of the enzyme for anti-VEGFA therapy. Methods: Mammalian cells (HEK293FT) or mouse retina were treated with shRNA, CasRx or Cas13bt3 carrying VEGFA targeting sgRNA. Total RNA was collected for RNA seqeuencing to study VEGFA mRNA expression and well as off-target genes. Results: Significant knockdown of VEGFA mRNA was observed with no effect on predicted off-target genes in mammalina cells and mouse retina. No loss of mouse VEGFA was also detected. Conclusions: AAV2.7m8 can be used for retina-wide transduction, and Cas13bt3 may be a potential new tool for control of VEGFA.
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| Overall design |
Examination of Cas13bt3 efficiency and transduction and anti-VEGFA potential in mammalian cells and mouse retinae.
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| Contributor(s) |
Guei-Shueng L |
| Citation missing |
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| BioProject |
PRJNA1077071 |
| |
| Submission date |
Feb 26, 2024 |
| Last update date |
Mar 02, 2024 |
| Contact name |
Guei-Sheung Liu |
| E-mail(s) |
rickliulab@gmail.com
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| Organization name |
Centre for Eye Research Australia
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| Street address |
32 Gisborne Street
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| City |
Melbourne |
| ZIP/Postal code |
3002 |
| Country |
Australia |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (61)
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