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Series GSE259220 Query DataSets for GSE259220
Status Public on Mar 01, 2024
Title Cas13bt3 knockdown of VEGFA mRNA in mammalian cells and mouse retina [RNA-Seq]
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: We report expression of a new Cas13 enzyme in retinal cells and evaluate its efficacy in targeting the VEGFA mRNA to establish safety and efficacy of the enzyme for anti-VEGFA therapy. Methods: Mammalian cells (HEK293FT) or mouse retina were treated with shRNA, CasRx or Cas13bt3 carrying VEGFA targeting sgRNA. Total RNA was collected for RNA seqeuencing to study VEGFA mRNA expression and well as off-target genes. Results: Significant knockdown of VEGFA mRNA was observed with no effect on predicted off-target genes in mammalina cells and mouse retina. No loss of mouse VEGFA was also detected. Conclusions: AAV2.7m8 can be used for retina-wide transduction, and Cas13bt3 may be a potential new tool for control of VEGFA.
 
Overall design Examination of Cas13bt3 efficiency and transduction and anti-VEGFA potential in mammalian cells and mouse retinae.
 
Contributor(s) Guei-Shueng L
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BioProject PRJNA1077071
Submission date Feb 26, 2024
Last update date Mar 02, 2024
Contact name Guei-Sheung Liu
E-mail(s) rickliulab@gmail.com
Organization name Centre for Eye Research Australia
Street address 32 Gisborne Street
City Melbourne
ZIP/Postal code 3002
Country Australia
 
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (61)
GSM8111555 batch1_hs_XNT-1
GSM8111556 batch1_hs_XNT-2
GSM8111557 batch1_hs_XNT-3

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE259220_RAW.tar 105.4 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA

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