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Series GSE25867 Query DataSets for GSE25867
Status Public on Dec 07, 2010
Title Expression data from DU145 cells treated with STAT3 siRNA
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Activation of Signal Transducer and Activator of Transcription 3 (STAT3) is common in prostate cancers. STAT3 may induce cell proliferation and resistance to apoptosis, as well as promote tumor angiogenesis, invasion, and migration by activating gene expression. Many STAT3-dependent transcriptional responses are mediated through protein-protein interactions that involve the amino-terminal domain (N-domain).
In this study, we found that inhibition of the STAT3 N-domain using novel inhibitor ST3-Hel2A-2 induces apoptotic death in prostate cancer cells. The cell death was accomponied by robust activation of pro-apoptotic gene. Using chromatin immunoprecipitation and tiling human promoter arrays (ChIP-chip), we have defined genome-wide targets of STAT3 in DU145 prostate cancer cells. We found that upregulated pro-apoptotic genes were bound by STAT3 in prostate cancer cells, and that STAT3 binding was decreased following inhibition of the STAT3 N-domain. STAT3 siRNA knockdow confirmed specificity of STAT3 binding and changes in gene expression.
 
Overall design DU145 cells were treated with STAT3 siRNA or scrambled siRNA for 48hr. Total RNA has been extracted and prepared for hybridization on Affymetrix HG-U133A 2.0 arrays.
 
Contributor(s) Timofeeva OA
Citation(s) 23288901
Submission date Dec 06, 2010
Last update date Dec 06, 2018
Contact name Olga A Timofeeva
E-mail(s) oat@georgetown.edu
Phone 202-687-8810
Fax 202-687-2221
Organization name Georgetown University Medical Center
Department Oncology
Street address 3900 Reservoir Rd NW
City Washington
State/province DC
ZIP/Postal code 20007
Country USA
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM635125 DU145 control (non-specific siRNA) 1
GSM635126 DU145 control (non-specific siRNA) 2
GSM635127 DU145 STAT3 siRNA-1
This SubSeries is part of SuperSeries:
GSE25944 Role of STAT3 in DU145 prostate cancer cell line
Relations
BioProject PRJNA142469

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25867_RAW.tar 7.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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