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Series GSE255686 Query DataSets for GSE255686
Status Public on May 28, 2024
Title Nociceptor-immune interactomes reveal insult-specific immune signatures of pain
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Inflammatory pain associated with tissue injury and infection largely results from the heightened sensitivity of the peripheral terminals of nociceptor sensory neurons as a consequence of exposure to inflammatory mediators. Targeting immune-derived inflammatory ligands, such as prostaglandin E2, reduces inflammatory pain, but more effective and safer therapies are needed. However, the diversity of immune cell and sensory neuron types, their ligands, and receptors make it challenging to map the immune mechanisms that contribute to inflammatory pain. We, therefore, used single-cell transcriptomics to explore which specific immune cell types affect the development of pain in diverse inflammatory pain models. We found that both the magnitude of macrophage and neutrophil recruitment and the injury-triggered transcriptional program in Cx3cr1 high and MHCII+ dermal macrophages closely mirror the kinetics of inflammatory pain hypersensitivity. We constructed a comprehensive list of potential cell-cell interactions covering receptors, ligands, and metabolites, and generated injury-specific neuroimmune interactomes based on immune cell and sensory neuron single-cell transcriptomic data. This analysis revealed both interactions common to multiple inflammatory pain models and those specific to a particular condition and uncovered immune mediators not previously identified as pain modulators.
 
Overall design CD45+ immune cells were FACS sorted cells from mouse (females) plantar skin at time 4h, 24, and 48h post zymosan injection, skin incision and UV burn as well as contra lateral healthy control. Gene expression profiles of immune cells was obtained at single cell level using 10x genomics platform.
Web link https://www.nature.com/articles/s41590-024-01857-2
 
Contributor(s) Jain A, Woolf C
Citation(s) 38806708
Submission date Feb 13, 2024
Last update date Jun 28, 2024
Contact name Riki Kawaguchi
Organization name University of California, Los Angeles
Department Department of Psychiatry and Neurology Department of Psychiatry
Lab AMRF Functional Genomics Common Research Resource
Street address 760 Westwood Plaza, Room 37-420
City Los Angeles
State/province CA
ZIP/Postal code 90095-1759
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM8077728 zymosan, contra, replicate 1
GSM8077729 zymosan, 4h, replicate 1
GSM8077730 zymosan, 24h, replicate 1
Relations
BioProject PRJNA1076164

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Supplementary file Size Download File type/resource
GSE255686_RAW.tar 1.7 Gb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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