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Status |
Public on May 28, 2024 |
Title |
Nociceptor-immune interactomes reveal insult-specific immune signatures of pain |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Inflammatory pain associated with tissue injury and infection largely results from the heightened sensitivity of the peripheral terminals of nociceptor sensory neurons as a consequence of exposure to inflammatory mediators. Targeting immune-derived inflammatory ligands, such as prostaglandin E2, reduces inflammatory pain, but more effective and safer therapies are needed. However, the diversity of immune cell and sensory neuron types, their ligands, and receptors make it challenging to map the immune mechanisms that contribute to inflammatory pain. We, therefore, used single-cell transcriptomics to explore which specific immune cell types affect the development of pain in diverse inflammatory pain models. We found that both the magnitude of macrophage and neutrophil recruitment and the injury-triggered transcriptional program in Cx3cr1 high and MHCII+ dermal macrophages closely mirror the kinetics of inflammatory pain hypersensitivity. We constructed a comprehensive list of potential cell-cell interactions covering receptors, ligands, and metabolites, and generated injury-specific neuroimmune interactomes based on immune cell and sensory neuron single-cell transcriptomic data. This analysis revealed both interactions common to multiple inflammatory pain models and those specific to a particular condition and uncovered immune mediators not previously identified as pain modulators.
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Overall design |
CD45+ immune cells were FACS sorted cells from mouse (females) plantar skin at time 4h, 24, and 48h post zymosan injection, skin incision and UV burn as well as contra lateral healthy control. Gene expression profiles of immune cells was obtained at single cell level using 10x genomics platform.
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Web link |
https://www.nature.com/articles/s41590-024-01857-2
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Contributor(s) |
Jain A, Woolf C |
Citation(s) |
38806708 |
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Submission date |
Feb 13, 2024 |
Last update date |
Jun 28, 2024 |
Contact name |
Riki Kawaguchi |
Organization name |
University of California, Los Angeles
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Department |
Department of Psychiatry and Neurology Department of Psychiatry
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Lab |
AMRF Functional Genomics Common Research Resource
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Street address |
760 Westwood Plaza, Room 37-420
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City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-1759 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (24)
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GSM8077731 |
zymosan, 48h, replicate 1 |
GSM8077732 |
zymosan, contra, replicate 2 |
GSM8077733 |
zymosan, 4h, replicate 2 |
GSM8077734 |
zymosan, 24h, replicate 2 |
GSM8077735 |
zymosan, 48h, replicate 2 |
GSM8077736 |
incision, contra, replicate 1 |
GSM8077737 |
incision, 4h, replicate 1 |
GSM8077738 |
incision, 24h, replicate 1 |
GSM8077739 |
incision, 48h, replicate 1 |
GSM8077740 |
incision, contra, replicate 2 |
GSM8077741 |
incision, 4h, replicate 2 |
GSM8077742 |
incision, 24h, replicate 2 |
GSM8077743 |
incision, 48h, replicate 2 |
GSM8077744 |
uvb, contra, replicate 17 |
GSM8077745 |
uvb, contra, replicate 18 |
GSM8077746 |
uvb, contra, replicate 19 |
GSM8077747 |
uvb, contra, replicate 20 |
GSM8077748 |
uvb, contra, replicate 21 |
GSM8077749 |
uvb, contra, replicate 22 |
GSM8077750 |
uvb, contra, replicate 23 |
GSM8077751 |
uvb, contra, replicate 24 |
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Relations |
BioProject |
PRJNA1076164 |
Supplementary file |
Size |
Download |
File type/resource |
GSE255686_RAW.tar |
1.7 Gb |
(http)(custom) |
TAR (of TAR) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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