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Series GSE2555 Query DataSets for GSE2555
Status Public on Oct 19, 2005
Title HCaRG-9 vs NEO-1
Organism Homo sapiens
Experiment type Expression profiling by array
Summary HEK293 cells were transfected with control plasmid (pcDNAI/Neo;Invitrogen) or with the plasmid encoding HCaRG. Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen).

Chips HG-U133A:
- Labeling protocol: Enzo BioArray HighYield RNA Transcript Labeling Kit.
- Hybridization: According to the manufacturer's protocol (Affymetrix).
- Scanner: GeneArray 2500.
- Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.

Chips HG-U133 Plus 2.0:
- Labeling protocol: GeneChip IVT Labeling Kit.
- Hybridization: According to the manufacturer's protocol (Affymetrix).
- Scanner: GeneChip Scanner 3000.
- Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.
Keywords: parallel sample
 
 
Contributor(s) El Hader C, Tremblay S, Solban N, Gingras D, Béliveau R, Orlov S, Hamet P, Tremblay J
Citation(s) 16033922
Submission date Apr 21, 2005
Last update date Mar 25, 2019
Contact name Johanne Tremblay
E-mail(s) johanne.tremblay@umontreal.ca
Phone 514-890-8000-12721
Organization name CHUM Research Center, Hotel-Dieu
Street address
City Montreal
State/province QUE
ZIP/Postal code H2W 1T8
Country Canada
 
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM48558 Neo-1 Ctl HG-U133A
GSM48669 HCaRG-9 HG-U133A
GSM48671 Neo-1 Ctl HG-U133 Plus 2.0
Relations
BioProject PRJNA92165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE2555_RAW.tar 22.7 Mb (http)(custom) TAR (of CEL)

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