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| Status |
Public on Mar 07, 2024 |
| Title |
The three-dimensional structure of chromatin condensed by PRC1 is porous and accessible [ATAC_CP15] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The compaction of chromatin is a prevalent paradigm in gene repression. Chromatin compaction is commonly thought to repress transcription by restricting chromatin accessibility. However, the spatial organisation and dynamics of chromatin compacted by gene-repressing factors are unknown. Using cryo-electron tomography, we solved the three-dimensional structure of chromatin condensed by the Polycomb Repressive Complex 1 (PRC1) in a complex with CBX8. Unexpectedly, PRC1-condensed chromatin is porous and forms a size-selective diffusion barrier that is stabilised through multivalent dynamic interactions. Accordingly, PRC1 remains dynamic within the condensate while maintaining the chromatin static. In differentiated mouse embryonic stem cells, CBX8-bound chromatin remains accessible. These findings challenge the idea of open and closed chromatin states and instead suggest that PRC1 permits size-selective accessibility of macromolecules into chromatin condensates.
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| Overall design |
ATAC-seq for mouse embryonic stem cells that have a TetO-GFP reporter gene cassette integrated into chromosome 15 and a TetR-CBX8 construct constitutively expressed. Cells were treated with dox to induce eviction of TetR-CBX8 from the TetO array
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| Contributor(s) |
Uckelmann M, Davidovich C |
| Citation(s) |
38405976 |
| |
| Submission date |
Feb 02, 2024 |
| Last update date |
Mar 08, 2024 |
| Contact name |
Michael Uckelmann |
| E-mail(s) |
michael.uckelmann@monash.edu
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| Organization name |
Monash University
|
| Department |
Biochemistry
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| Lab |
Davidovich
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| Street address |
23 Innovation Walk
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| City |
Clayton |
| State/province |
Victoria |
| ZIP/Postal code |
3800 |
| Country |
Australia |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE220140 |
The three-dimensional structure of chromatin condensed by PRC1 is porous and accessible |
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| Relations |
| BioProject |
PRJNA1072731 |
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