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Series GSE254602 Query DataSets for GSE254602
Status Public on Mar 21, 2024
Title Effect of deletion of SPTLC1 on gene expression and differentiation of mouse Th17 cells in vitro
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Th17 cells are implicated in autoimmune diseases and several metabolic processes are shown to be important for their development and function. However, the role of de novo sphingolipid biosynthesis in their differentiation and function is less known. To investigate the role of SPTLC1(major subunit of serine palmitoyl transferase enzyme (SPT) that catalyzes the first and rate limiting step of de novo sphingolipid synthesis) in mouse Th17 cell differentiation, we have activated the WT and SPTLC1 defecient (KO) naïve CD4+ T cells, isolated from mouse spleen and lymph nodes, in Th17 differentiating condition in the precence and absence of NAC ( N-acetyl cysteine) and 3-KDS (3-ketodihydrosphingosine).
 
Overall design To investigate the role of SPTLC1 in Th17 differentiation, total RNA was extracted for each of the 3 biological replicates from WT and KO naive CD4+ T cells and Th17 cells. To understand the specificity of the de novo sphingolipid biosynthetic pathway in the observed effect KO Th17 cells, RNA was isolated from WT and KO naive CD4+T cells cultured under Th17 polarizing condition in the presence and absense of 3-KDS (product of SPT enzyme). Further, to understand role of ROS in the observed defect in KO Th17 cells, RNA was isolated from WT and KO naive CD4+T cells cultured under Th17 polarizing condition in the presence and absense of 3-KDS (product of SPT enzyme)
 
Contributor(s) Abimannan T, Acharya U, Acharya JK
Citation(s) 38657065
Submission date Jan 30, 2024
Last update date May 10, 2024
Contact name Thiruvaimozhi Abimannan
E-mail(s) thiruvaimozhi.abimannan@nih.gov
Phone 3018465652
Organization name National Cancer Institute
Department CDBL
Lab Jairaj Acharya Lab (SPHINGOLIPID AND PHOSPHOLIPID SIGNALING SECTION)
Street address Building 560, Room 22-6
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM8046908 WT Th17 biol rep 1
GSM8046909 WT Th17 biol rep 2
GSM8046910 WT Th17 biol rep 3
Relations
BioProject PRJNA1071114

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE254602_gene_count.xls.gz 2.6 Mb (ftp)(http) XLS
GSE254602_gene_fpkm.xls.gz 6.0 Mb (ftp)(http) XLS
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