|
| Status |
Public on Jan 25, 2024 |
| Title |
Scd-1 deficiency promotes the differentiation of CD8+ T effector |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The impact of various fatty acid types on adaptive immunity remains uncertain, and their roles remain unelucidated. In this study, we showed that the enzyme stearoyl-CoA desaturase-1 (Scd-1) and its resulting compound, oleic acid (OA), have a substantial impact on the transformation of CD8+ naïve T cells into effector T cells. Inactivation of Scd-1 triggers the specialization of CD8+ T cells into the Teff subset, enhancing the effector function and mitochondrial metabolism of Teff cells, and OA can partially counteract this.
|
| |
|
| Overall design |
To further explore the influence of Scd-1 on the differentiation and functional status of Teffs, we isolated CD8+ T cells from the spleens of both WT and Scd-1-/- mice using MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend), which were then induced to differentiate into Teff cells using IL-2 after three days of stimulation with CD3/28.
We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells.
Comparative gene expression profiling analysis of RNA-seq data for CD8+ Teff cells from WT and Scd-1-/- mice.
|
| |
|
| Contributor(s) |
Lin Y, Li X |
| Citation(s) |
38379772 |
| |
| Submission date |
Jan 20, 2024 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Yiwei Lin |
| E-mail(s) |
linyiwei2018@gmail.com
|
| Organization name |
Renji Hospital, Shanghai Jiao Tong University School of Medicine
|
| Street address |
No.1630 Dongfang Road, Shanghai
|
| City |
Shanghai |
| ZIP/Postal code |
200127 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1067100 |
Less...
More...