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Series GSE253607 Query DataSets for GSE253607
Status Public on Jan 25, 2024
Title 24 h differentiation in N2B27 of wild-type and Med12-mutant mESCs [bulkRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cell differentiation results from coordinated changes in gene transcription in response to combinations of signals. FGF, Wnt, and mTOR signals regulate the differentiation of pluripotent mammalian cells towards embryonic and extraembryonic lineages, but how these signals cooperate with general transcriptional regulators is not fully resolved. Here, we report a genome-wide CRISPR screen that reveals both signaling components and general transcriptional regulators for differentiation-associated gene expression in mESCs. Focusing on the Mediator subunit Med12 as one of the strongest hits in the screen, we show that it regulates gene expression in parallel to FGF and mTOR signals. Loss of Med12 is compatible with differentiation along both the embryonic epiblast and the extraembryonic primitive endoderm lineage, but pluripotency transitions are slowed down, and the transcriptional separation between epiblast and primitive endoderm identities is enhanced in Med12-mutant cells. These cellular phenotypes correlate with reduced biological noise upon loss of Med12. These findings suggest that Med12 regulates cellular plasticity through the priming of transcriptional changes during differentiation, thereby modulating the effects of a broad range of signals.
 
Overall design Gene expression in wild-type and Med12 mutant mESCs was compared in pluripotency and after 24h of differentiation in N2B27. Triplicates were obtained by seeding different passages on different days
 
Contributor(s) Fernkorn M, Schröter C
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Submission date Jan 18, 2024
Last update date Jan 25, 2024
Contact name Max Fernkorn
E-mail(s) max.fernkorn@mpi-dortmund.mpg.de
Organization name Max Planck Institute of Molecular Physiology
Department Systemic Cell Biology
Street address Otto-Hahn-Strasse 11
City Dortmund
ZIP/Postal code 44225
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM8024043 wt, 2i, rep1
GSM8024044 wt, 2i, rep2
GSM8024045 wt, 2i, rep3
This SubSeries is part of SuperSeries:
GSE253609 Med12 cooperates with multiple differentiation signals to enhance embryonic stem cell plasticity
Relations
BioProject PRJNA1066346

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Supplementary file Size Download File type/resource
GSE253607_All_Probes_Log2TPMs.txt.gz 3.5 Mb (ftp)(http) TXT
GSE253607_All_Probes_Raw_Counts.txt.gz 1.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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