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Series GSE253581 Query DataSets for GSE253581
Status Public on May 29, 2024
Title Transcriptional profiling of CD11b+ microglia isolated from cerebellum and olfactory bulb-removed brains of K18-seeded P301L, PBS-injected P301L and PBS-injected FVB at 1-week, 1-month, and 3-months post-injection.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To gain deeper insights into the relationship between Tau spreading and microglial response, we conducted a comprehensive analysis of microglial heterogeneity in K18-seeded P301L mice at 1 week, 1 month, and 3 months post-injection. We compared these findings with those from control groups: P301L mice injected with PBS and their non-transgenic counterparts, the FVB mice also subjected to PBS injection. For this purpose, we employed CITE-seq to investigate CD11b positive cells isolated from cerebellum and olfactory bulb-removed brains.
Overall design P301L mice represent a transgenic lineage originating from FVB mice, characterized by their expression of the human tau protein, specifically the 4R/2N isoform. At the age of 3 months, both the transgenic P301L mice and their non-transgenic FVB counterparts were injected with either PBS or K18. Subsequently, the brains of K18-seeded P301L, PBS-injected P301L, and PBS-injected FVB mice were extracted at 1-week, 1-month, and 3-month time points post-injection. After removing the cerebellum and olfactory bulbs, the remaining brain tissue was physically and enzymatically dissociated in the presence of Actinomycin D and Brefeldin A. Following dissociation, CITE-seq antibodies (ADTs and HTOs) and CD11b-PE/Cy5 staining were used. The DAPI-CD11b+ cells were subsequently isolated using fluorescence-activated cell sorting (FACS).
Contributor(s) Gruel R, Bijnens B, Van Den Daele J, Thys S, Willems R, Wuyts D, Roels J, Mancuso R, Pita-Almenar JD, Vandamme N, De Vos WH
Citation(s) 38403918
Submission date Jan 18, 2024
Last update date May 29, 2024
Contact name Jana Roels
Organization name VIB
Department VIB Single cell core
Street address Technologiepark-Zwijnaarde 71
City Zwijnaarde
ZIP/Postal code 9052
Country Belgium
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (27)
GSM8023809 1-week PI PBS-injected FVB, GEX
GSM8023810 1-week PI PBS-injected FVB, ADT
GSM8023811 1-week PI PBS-injected FVB,HTO
BioProject PRJNA1066313

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Supplementary file Size Download File type/resource
GSE253581_P301L.rds.gz 1.7 Gb (ftp)(http) RDS
GSE253581_P301L.xlsx 85.8 Kb (ftp)(http) XLSX
GSE253581_RGR005_raw_feature_bc_matrix.h5 52.4 Mb (ftp)(http) H5
GSE253581_RGR006_raw_feature_bc_matrix.h5 48.5 Mb (ftp)(http) H5
GSE253581_RGR007_raw_feature_bc_matrix.h5 34.7 Mb (ftp)(http) H5
GSE253581_RGR010_raw_feature_bc_matrix.h5 78.4 Mb (ftp)(http) H5
GSE253581_RGR011_raw_feature_bc_matrix.h5 91.5 Mb (ftp)(http) H5
GSE253581_RGR012_raw_feature_bc_matrix.h5 118.9 Mb (ftp)(http) H5
GSE253581_RGR013_raw_feature_bc_matrix.h5 69.3 Mb (ftp)(http) H5
GSE253581_RGR014_raw_feature_bc_matrix.h5 74.2 Mb (ftp)(http) H5
GSE253581_RGR015_raw_feature_bc_matrix.h5 110.2 Mb (ftp)(http) H5
GSE253581_readme.txt 4.8 Kb (ftp)(http) TXT
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