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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 29, 2024 |
| Title |
Transcriptional profiling of CD11b+ microglia isolated from cerebellum and olfactory bulb-removed brains of K18-seeded P301L, PBS-injected P301L and PBS-injected FVB at 1-week, 1-month, and 3-months post-injection. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
To gain deeper insights into the relationship between Tau spreading and microglial response, we conducted a comprehensive analysis of microglial heterogeneity in K18-seeded P301L mice at 1 week, 1 month, and 3 months post-injection. We compared these findings with those from control groups: P301L mice injected with PBS and their non-transgenic counterparts, the FVB mice also subjected to PBS injection. For this purpose, we employed CITE-seq to investigate CD11b positive cells isolated from cerebellum and olfactory bulb-removed brains.
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| Overall design |
P301L mice represent a transgenic lineage originating from FVB mice, characterized by their expression of the human tau protein, specifically the 4R/2N isoform. At the age of 3 months, both the transgenic P301L mice and their non-transgenic FVB counterparts were injected with either PBS or K18. Subsequently, the brains of K18-seeded P301L, PBS-injected P301L, and PBS-injected FVB mice were extracted at 1-week, 1-month, and 3-month time points post-injection. After removing the cerebellum and olfactory bulbs, the remaining brain tissue was physically and enzymatically dissociated in the presence of Actinomycin D and Brefeldin A. Following dissociation, CITE-seq antibodies (ADTs and HTOs) and CD11b-PE/Cy5 staining were used. The DAPI-CD11b+ cells were subsequently isolated using fluorescence-activated cell sorting (FACS).
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| Contributor(s) |
Gruel R, Bijnens B, Van Den Daele J, Thys S, Willems R, Wuyts D, Roels J, Mancuso R, Pita-Almenar JD, Vandamme N, De Vos WH |
| Citation(s) |
38403918 |
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| Submission date |
Jan 18, 2024 |
| Last update date |
May 29, 2024 |
| Contact name |
Jana Roels |
| E-mail(s) |
jana.roels@vib.be, niels.vandamme@vib.be
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| Organization name |
VIB
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| Department |
VIB Single cell core
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| Street address |
Technologiepark-Zwijnaarde 71
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| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (27)
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| GSM8023812 |
1-week PI PBS-injected P301L, GEX |
| GSM8023813 |
1-week PI PBS-injected P301L, ADT |
| GSM8023814 |
1-week PI PBS-injected P301L,HTO |
| GSM8023815 |
1-week PI K18-seeded P301L, GEX |
| GSM8023816 |
1-week PI K18-seeded P301L, ADT |
| GSM8023817 |
1-week PI K18-seeded P301L,HTO |
| GSM8023818 |
1-month PI PBS-injected FVB, GEX |
| GSM8023819 |
1-month PI PBS-injected FVB, ADT |
| GSM8023820 |
1-month PI PBS-injected FVB,HTO |
| GSM8023821 |
1-month PI PBS-injected P301L, GEX |
| GSM8023822 |
1-month PI PBS-injected P301L, ADT |
| GSM8023823 |
1-month PI PBS-injected P301L,HTO |
| GSM8023824 |
1-month PI K18-seeded P301L, GEX |
| GSM8023825 |
1-month PI K18-seeded P301L, ADT |
| GSM8023826 |
1-month PI K18-seeded P301L,HTO |
| GSM8023827 |
3-months PI PBS-injected FVB, GEX |
| GSM8023828 |
3-months PI PBS-injected FVB, ADT |
| GSM8023829 |
3-months PI PBS-injected FVB,HTO |
| GSM8023830 |
3-months PI PBS-injected P301L, GEX |
| GSM8023831 |
3-months PI PBS-injected P301L, ADT |
| GSM8023832 |
3-months PI PBS-injected P301L,HTO |
| GSM8023833 |
3-months PI K18-seeded P301L, GEX |
| GSM8023834 |
3-months PI K18-seeded P301L, ADT |
| GSM8023835 |
3-months PI K18-seeded P301L,HTO |
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| Relations |
| BioProject |
PRJNA1066313 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE253581_P301L.rds.gz |
1.7 Gb |
(ftp)(http) |
RDS |
| GSE253581_P301L.xlsx |
85.8 Kb |
(ftp)(http) |
XLSX |
| GSE253581_RGR005_raw_feature_bc_matrix.h5 |
52.4 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR006_raw_feature_bc_matrix.h5 |
48.5 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR007_raw_feature_bc_matrix.h5 |
34.7 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR010_raw_feature_bc_matrix.h5 |
78.4 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR011_raw_feature_bc_matrix.h5 |
91.5 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR012_raw_feature_bc_matrix.h5 |
118.9 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR013_raw_feature_bc_matrix.h5 |
69.3 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR014_raw_feature_bc_matrix.h5 |
74.2 Mb |
(ftp)(http) |
H5 |
| GSE253581_RGR015_raw_feature_bc_matrix.h5 |
110.2 Mb |
(ftp)(http) |
H5 |
| GSE253581_readme.txt |
4.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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