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| Status |
Public on Mar 23, 2024 |
| Title |
Characterization of the role of SETDB1 in Muscle stem cells during skeletal muscle regeneration [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We are characterizing the role of an H3K9 histone methyltransferase. As these proteins have the capacity to modulate chromatin, we investigate the chromatin modulation using H3K9Me3 Chip-seq.
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| Overall design |
Muscle Stem Cells were isolated from the conditional mice model Setdb1-cKO and were isolated by Magnetic-activated cell sorting (MACS) and cultured in vitro. We treated the cells with either Ethanol or 4-hydroxytamoxifen in order to induce Setdb1 deletion and after 72 hours of treatment performed the H3K9Me3 Chip-Seq.
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| Contributor(s) |
Le Grand F, Garcia P, Valentin P, Slimane A |
| Citation missing |
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| |
| Submission date |
Jan 17, 2024 |
| Last update date |
Mar 23, 2024 |
| Contact name |
Pauline GARCIA |
| E-mail(s) |
garcia.pauline02@gmail.com
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| Organization name |
INMG
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| Lab |
PGNM
|
| Street address |
8 avenue Rockefeller
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| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1065801 |
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