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| Status |
Public on Apr 24, 2024 |
| Title |
scRNA-seq of day 32 p.i. GC B cells induced by experimental Plasmodium infection following the inducible deletion of IL-21 from CD4 T cells on day 16-18 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
IL-21 is critical for initiating humoral immune responses, but whether sustained IL-21 production by T follicular helper cells regulates the quality or output of memory B cells and plasma cells during an established germinal center response is unknown. Here, we applied scRNA and approaches to determine the heterogeneity of germinal center B cells from Plasmodium infected mice with or without truncated IL-21 expression.
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| Overall design |
Mice were infected with 1 million Plasmodium yoelii parasitized red blood cells and subsequently treated with tamoxifen on days 16, 17 and 18 p.i. to induce the deletion of Il21 from the CD4 T cell compartment. Comparator cells derive from Plasmodium infected and tamoxifen treated mice bearing the conditional alleles of Il21 but lacking the Cd4-ERT2/Cre transgene. On day 32 p.i., CD95+ GL-7+CD19+B220+ cells were FACS-sorted from mice (4 donor mice/genotype) and loaded on the Chromium Controller (10x Genomics) Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3’ v2 Reagent Kit (10x Genomics) according to the manufacturer’s protocol.
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| Contributor(s) |
Johnson JT, Butler NS |
| Citation(s) |
38477614 |
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| Submission date |
Jan 16, 2024 |
| Last update date |
Apr 25, 2024 |
| Contact name |
Noah S Butler |
| E-mail(s) |
noah-butler@uiowa.edu
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| Organization name |
University of Iowa
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| Department |
Department of Microbiology and Immunology
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| Lab |
Butler Lab
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| Street address |
4-350 BSB, 51 Newton Road
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| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1065457 |
