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Series GSE253100 Query DataSets for GSE253100
Status Public on Feb 01, 2024
Title Quantitative live-cell imaging of secretion activity reveals dynamic immune responses
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Quantification of cytokine secretion has facilitated advances in the field of immunology, yet the dynamic and varied secretion profiles of individual cells, particularly those obtained from limited human samples, remain obscure. Herein, we introduce a new technology for quantitative live-cell imaging of secretion activity (qLCI-S) that enables high-throughput and dual-color monitoring of secretion activity at the single-cell level over several days, followed by transcriptome analysis of individual cells based on their phenotype. The efficacy of qLCI-S was demonstrated by visualizing the characteristic temporal pattern of cytokine secretion of group 2 innate lymphoid cells, which constitute less than 0.01% of human peripheral blood mononuclear cells, and by revealing minor subpopulations with enhanced cytokine production. The underlying mechanism of this feature was linked to the gene expression of stimuli receptors. This new technology paves the way for exploring gene expression signatures linked to the spatiotemporal dynamic nature of various secretory functions.
Overall design After 5-days of qLCI-S for the ILC2s stimulated with IL-2/IL-33/TSLPs, hyperactive and silent phenotypes were harvested as individual clones and single cells, respectively, and applied to RNA-Seq analysis.
Contributor(s) Shirasaki Y, Yamagishi M, Miyata K, Tanaka Y, Ohara O, Fukunaga K, Moro K, Uemura S
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Submission date Jan 12, 2024
Last update date Feb 01, 2024
Contact name Yoshitaka Shirasaki
Phone +81-3-5841-4761
Organization name The University of Tokyo
Department Graduate School of Pharmaceutical Science
Lab Laboratory of Bioanalytical Chemistry
Street address Hongo 7-3-1
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (7)
GSM8013680 peripheral blood, N1
GSM8013681 peripheral blood, N2
GSM8013682 peripheral blood, N3
BioProject PRJNA1064034

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Supplementary file Size Download File type/resource
GSE253100_RAW.tar 570.0 Kb (http)(custom) TAR (of CSV)
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Raw data are available in SRA

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