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GEO help: Mouse over screen elements for information. |
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Status |
Public on Oct 09, 2024 |
Title |
Identification of genes bound by NFAT5-SMADs complex |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Oncogenic KRAS is now considered a druggable target; however, multiple mechanisms contribute to the development of resistance to KRAS-targeted therapy. A significant factor in therapy resistance is the alteration in cell state or cellular plasticity, exemplified by the epithelial-to-mesenchymal transition (EMT) phenotype. In pancreatic ductal adenocarcinoma (PDAC), the negative correlation between addiction to oncogenic KRAS signaling and EMT has been observed, yet the role of cell plasticity and its underlying mechanisms in governing resistance remain unclear. Our findings reveal that the pivotal EMT driver, TGFβ, facilitates KRAS bypass in PDAC through the nuclear factor NFAT5. NFAT5 interacts with canonical TGFβ factors SMAD3 and SMAD4, inducing EMT and therapy resistance. To identify DNA bound by the NFAT5-SMADs complex, we conducted Chromatin IP followed by next-generation sequencing (ChIP-seq) using antibodies binding to NFAT5, SMAD2, SMAD3, and SMAD4.
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Overall design |
We conducted ChIP-seq using antibodies binding to endogenous NFAT5, SMAD2, SMAD3, and SMAD4 in mouse PDAC cells.
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Contributor(s) |
Hou P, Deng D |
Citation(s) |
39432061 |
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Submission date |
Jan 09, 2024 |
Last update date |
Jan 08, 2025 |
Contact name |
Pingping Hou |
E-mail(s) |
ph413@njms.rutgers.edu
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Organization name |
Rutgers University
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Department |
Microbiology, Biochemistry and Molecular Genetics
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Lab |
Pingping Hou Lab
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Street address |
205 South Orange Ave
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City |
Newark |
State/province |
New Jersey |
ZIP/Postal code |
07103 |
Country |
USA |
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Platforms (1) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE252839 |
Nuclear Factor NFAT5 Governs Cellular Plasticity-Induced KRAS-Targeted Therapy Resistance in Pancreatic Cancer |
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Relations |
BioProject |
PRJNA1062799 |
Supplementary file |
Size |
Download |
File type/resource |
GSE252838_RAW.tar |
2.8 Gb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
Raw data are available in SRA |
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