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Series GSE252838 Query DataSets for GSE252838
Status Public on Oct 09, 2024
Title Identification of genes bound by NFAT5-SMADs complex
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Oncogenic KRAS is now considered a druggable target; however, multiple mechanisms contribute to the development of resistance to KRAS-targeted therapy. A significant factor in therapy resistance is the alteration in cell state or cellular plasticity, exemplified by the epithelial-to-mesenchymal transition (EMT) phenotype. In pancreatic ductal adenocarcinoma (PDAC), the negative correlation between addiction to oncogenic KRAS signaling and EMT has been observed, yet the role of cell plasticity and its underlying mechanisms in governing resistance remain unclear. Our findings reveal that the pivotal EMT driver, TGFβ, facilitates KRAS bypass in PDAC through the nuclear factor NFAT5. NFAT5 interacts with canonical TGFβ factors SMAD3 and SMAD4, inducing EMT and therapy resistance. To identify DNA bound by the NFAT5-SMADs complex, we conducted Chromatin IP followed by next-generation sequencing (ChIP-seq) using antibodies binding to NFAT5, SMAD2, SMAD3, and SMAD4.
 
Overall design We conducted ChIP-seq using antibodies binding to endogenous NFAT5, SMAD2, SMAD3, and SMAD4 in mouse PDAC cells.
 
Contributor(s) Hou P, Deng D
Citation(s) 39432061
Submission date Jan 09, 2024
Last update date Jan 08, 2025
Contact name Pingping Hou
E-mail(s) ph413@njms.rutgers.edu
Organization name Rutgers University
Department Microbiology, Biochemistry and Molecular Genetics
Lab Pingping Hou Lab
Street address 205 South Orange Ave
City Newark
State/province New Jersey
ZIP/Postal code 07103
Country USA
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (4)
GSM8008785 PDAC_SMAD2_ChIP
GSM8008786 PDAC_SMAD3_ChIP
GSM8008787 PDAC_SMAD4_ChIP
This SubSeries is part of SuperSeries:
GSE252839 Nuclear Factor NFAT5 Governs Cellular Plasticity-Induced KRAS-Targeted Therapy Resistance in Pancreatic Cancer
Relations
BioProject PRJNA1062799

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Supplementary file Size Download File type/resource
GSE252838_RAW.tar 2.8 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA

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