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Series GSE252397 Query DataSets for GSE252397
Status Public on Mar 21, 2024
Title Targeting the tissue factor coagulation initiation complex prevents antiphospholipid antibody development.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Antiphospholipid antibodies (aPL) in primary or secondary antiphospholipid syndrome (APS) are a major cause for acquired thrombophilia. Here we show that specific inhibition of the TF coagulation initiation complex with nematode anticoagulant protein c2 (NAPc2) prevents the the development of persistent aPL and circulating phospholipid-reactive B1 cells in latent viral infection and lupus prone MRL-lpr mice. NGS mRNA profiling of splenic DC in lupus mice treated with NAPc2 versus saline control uncovered a suppression of dendritic cell activation in the spleen.
Overall design NGS sequencing of splenic DCs used spleens digested with collagenase II and DNase I followed by dissociated cell enrichment on an Optiprep density gradient and FACS of live DCs identified by MHCII and CD11c in the CD3/CD19/NK1.1 negative population. DC RNA of 4 saline and 4 NAPc2 treated mice was sequenced by Novogene and mapped to the GRCm39 mouse genome with STAR on the fly.
Contributor(s) Wolfram R, Jens P, T Son N
Citation(s) 38142429
Submission date Jan 02, 2024
Last update date Jun 21, 2024
Contact name Wolfram Ruf
Organization name CTH Mainz
Street address Langenbeckstrasse 1
City Mainz
ZIP/Postal code 55131
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (10)
GSM8000764 Saline replicate 1
GSM8000765 Saline replicate 2
GSM8000766 Saline replicate 3
BioProject PRJNA1060333

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Supplementary file Size Download File type/resource
GSE252397_RAW.tar 2.5 Mb (http)(custom) TAR (of TAB)
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Raw data are available in SRA
Processed data provided as supplementary file

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