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Series GSE251800 Query DataSets for GSE251800
Status Public on Jan 25, 2024
Title mRNA-based precision targeting of neoantigens and tumor-associated antigens in malignant brain tumors [KR158 RNA-TCR]
Organism Mus musculus
Experiment type Other
Summary mRNA-based immunotherapeutics have garnered widespread interest for their potential to revolutionize cancer treatment. In this study, we have established a personalized and customizable mRNA-therapeutic approach that effectively targets a plurality of tumor antigens and demonstrated potent anti-tumor response in preclinical brain tumor models. Our platform uniquely addresses the challenge of tumor heterogeneity and low antigen burden, two key deficiencies in targeting classically immunotherapy-resistant CNS malignancies.
Overall design We created GBM tumor antigen-specific RNA and loaded it into dendritic cells for anti-tumor vaccine. We performed in vivo anti-tumor efficacy study with antigen loaded dendritic cells in combination with adoptive T cell therapy with relevant controls in the KR158 murine GBM model. Post therapy, we isolated the CD45+ve immune cells from the brain tumor microenvironment and performed TCR seq analysis from the RNA.
Contributor(s) Trivedi V, Yang C, Micthell D
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BioProject PRJNA1054323
Submission date Dec 21, 2023
Last update date Jan 26, 2024
Contact name Duane Mitchell
Organization name University of Florida
Street address 1333 Center Drive, BSB B1-150
City Gainesville
State/province Florida
ZIP/Postal code 32610
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (9)
GSM7988373 Untreated biol rep 2 [TCR-1]
GSM7988374 Untreated biol rep 3 [TCR-2]
GSM7988375 Untreated biol rep 6 [TCR-3]

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE251800_RAW.tar 670.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA

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