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Series GSE250338 Query DataSets for GSE250338
Status Public on Jan 30, 2024
Title GFP and mCherry gene expression at single cell level of all liver cell types from B6J mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Diacylglycerol acyltransferase (DGAT)-2 catalyzes the final step of triglyceride (TG) synthesis. DGAT2 deletion in mice lowers liver TGs and DGAT2 overexpression might have the opposite effect. Mouse DGAT2 was overexpressed in C57Bl/6J mice using adeno-associated virus 8 (AAV8 and AAV-DJ). The tissue specificity of AAV8 and AAV-DJ was first evaluated. To confirm that AAV8 and AAV-DJ only infected hepatocytes and not other cells in liver, we carried out single cell sequencing of liver cells isolated from mice infected with AAV8-mCherry and AAV-DJ-GFP. The mCherry and GFP signals were highly expressed in hepatocytes and not in stellate, endothelial, or Kupffer cells.
 
Overall design An 8-week-old C57BL/6J male mouse was injected retro-orbitally with an AAV-DJ virus that was engineered to express GFP and AAV-8 engineered to express mCherry. The mouse was fed a chow diet ad lib and sacrificed in 3 weeks after virus injection. Hepatocytes and non-parenchymal liver cells (NPCs) were isolated for the sc-RNA seq. New custom mice gene reference genomes were created by adding both GFP and mCherry into the existing mouse gene reference genomes. GFP and mCherry gene expression were assessed in both hepatocytes and NPCs.
 
Contributor(s) Wang S, Horton JD
Citation(s) 38340721
Submission date Dec 15, 2023
Last update date Mar 20, 2024
Contact name Simeng Wang
E-mail(s) Simeng.Wang@UTSouthwestern.edu
Phone 2037104281
Organization name UT Southwestern Medical Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (1)
GSM7977780 GFP_MCHERRY_scRNAseq
Relations
BioProject PRJNA1053384

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Supplementary file Size Download File type/resource
GSE250338_RAW.tar 968.3 Mb (http)(custom) TAR (of FA, FASTA, GTF, MTX, TSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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