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| Status |
Public on Dec 19, 2023 |
| Title |
sciMET-cap: High-throughput single-cell methylation analysis with a reduced sequencing burden |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
DNA methylation is a key component of the mammalian epigenome, playing a regulatory role in development, disease, and other processes. Robust, high-throughput single-cell DNA methylation assays are now possible (sciMET); however, the genome-wide nature of DNA methylation results in a high sequencing burden per cell. Here, we leverage target enrichment with sciMET to capture sufficient information per cell for cell type assignment using substantially fewer sequence reads (sciMET-cap). Sufficient off-target coverage further enables the production of near-complete methylomes for individual cell types. We characterize sciMET-cap on human PBMCs and brain (middle frontal gyrus).
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| Overall design |
Crypreserved commercially-obtained PBMCs were used for all data included in this data deposition
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| Contributor(s) |
Adey A |
| Citation(s) |
38987810 |
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| Submission date |
Dec 15, 2023 |
| Last update date |
Aug 08, 2024 |
| Contact name |
Andrew Adey |
| E-mail(s) |
adey@ohsu.edu
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| Organization name |
Oregon Health & Science University
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| Department |
MMG
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| Lab |
Adey Lab
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| Street address |
3181 S.W. Sam Jackson Park Rd, RHH, L103
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| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239-3098 |
| Country |
USA |
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| Platforms (1) |
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| Samples (5)
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| GSM7976776 |
sciMETcap on PBMCs, wash temperature increased to 67C |
| GSM7976777 |
sciMETcap 6k cells, standard capture conditions |
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| Relations |
| BioProject |
PRJNA1053297 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE250282_processed_data.tar.gz |
16.0 Gb |
(ftp)(http) |
TAR |
SRA Run Selector |
| Raw data are available in SRA |
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