NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE250084 Query DataSets for GSE250084
Status Public on Apr 04, 2024
Title Extraembryonic gut endoderm cells undergo programmed cell death during development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Despite a distinct developmental origin, extraembryonic cells in mice contribute to gut endoderm and converge to transcriptionally resemble their embryonic counterparts. Notably, extraembryonic progenitors share a non-canonical epigenome, raising several pertinent questions, including whether this landscape is reset to match the embryonic regulation and if these cells persist into later development. Here, we developed a two-color lineage tracing strategy to track and isolate extraembryonic cells over time. We find that extraembryonic gut cells display substantial memory of their developmental origin including retention of their original DNA methylation landscape and resulting transcriptional signatures. Furthermore, we show that extraembryonic gut cells undergo programmed cell death and neighboring embryonic cells clear their remnants via non-professional phagocytosis. By midgestation, we no longer detect extraembryonic cells in the wild type gut while they persist and differentiate further in p53 mutant embryos. Our study provides key insights into the molecular and developmental fate of extraembryonic cells inside the embryo.
 
Overall design Refer to individual Series
 
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 13, 2023
Last update date May 15, 2024
Contact name Sara Hetzel
E-mail(s) hetzel@molgen.mpg.de
Organization name Max Planck Institute for Molecular Genetics
Department Genome Regulation
Lab Meissner Lab
Street address Ihnestra├če 63
City Berlin
ZIP/Postal code 14195
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (88)
GSM6859078 scRNA_E95_mCherry_and_GFP_mCherry_dual
GSM7973610 RNAseq_E135_emColon_EPCAM_GFP_WT_Rep1
GSM7973611 RNAseq_E135_emColon_EPCAM_GFP_WT_Rep2
This SuperSeries is composed of the following SubSeries:
GSE221362 Extraembryonic gut endoderm cells undergo programmed cell death during development (scRNA-seq)
GSE250083 Extraembryonic gut endoderm cells undergo programmed cell death during development (RNA-seq)
GSE250180 Extraembryonic gut endoderm cells undergo programmed cell death during development (RRBS)
Relations
BioProject PRJNA1051994

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE250084_RAW.tar 3.1 Gb (http)(custom) TAR (of BW, TAR, TSV)
SRA Run SelectorHelp

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap