|
| Status |
Public on Apr 02, 2024 |
| Title |
Trascriptional profiling of proliferating and resting fracntions in ibrutinib-treated CLL |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bruton’s tyrosine kinase (BTK) inhibitors such as ibrutinib represent an effective strategy for treatment of chronic lymphocytic leukemia (CLL), although ~30% of patients eventually undergo disease progression. Here we investigated the long-term modulation of the CXCR4dim/CD5bright proliferative fraction (PF) and the CXCR4bright/CD5dim resting fraction (RF) in CLL samples, and their correlation with therapeutic outcome and emergence of ibrutinib resistance. Longitudinal tracking by flow cytometry revealed that PF, initially suppressed by ibrutinib, reappeared upon early disease progression suggesting that PF evaluation could represent a sensitive and specific marker of CLL progression upon ibrutinib treatment. Transcriptomic analyses of PF at progression revealed similar proliferation signatures between pre- and post-treatment PF, demonstrating the emergence upon progression of a newly proliferating cell population.
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| |
|
| Overall design |
PF and RF fractions were cell-sorted from thawed PBMCs and nuclei acids immediately purified. 30-50ng of RNA were processed using mRNA library prep kit
|
| Web link |
https://www.nature.com/articles/s41375-024-02301-y
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| |
|
| Contributor(s) |
Pozzo F, Gattei V, Bomben R, Vit F |
| Citation(s) |
38914716 |
| |
| Submission date |
Dec 12, 2023 |
| Last update date |
Jul 02, 2024 |
| Contact name |
Federico Pozzo |
| E-mail(s) |
federico.pozzo@cro.it
|
| Phone |
+390434659719
|
| Organization name |
Centro di Riferimento Oncologico (CRO) di Aviano IRCCS
|
| Department |
Department of Translational Research
|
| Lab |
Clinical and Experimental Onco Hematology Unit
|
| Street address |
via Franco Gallini 2
|
| City |
Aviano |
| State/province |
PN |
| ZIP/Postal code |
33081 |
| Country |
Italy |
| |
|
| Platforms (2) |
| GPL21697 |
NextSeq 550 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (40)
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| Relations |
| BioProject |
PRJNA1051488 |
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