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Series GSE24983 Query DataSets for GSE24983
Status Public on Oct 29, 2010
Title Response of A549 cells treated with Aspergillus fumigatus [WT-CF_vs_WT-GC]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Response of A549 cells treated with Aspergillus fumigatus germinating conidia (WT-GC) or culture filtrate (WT-CF) for 8h
Aspergillus fumigatus is the most commonly encountered mold pathogen of humans, predominantly infecting the respiratory system. Colonization and penetration of the lung alveolar epithelium is a key but poorly understood step in the infection process. This study focused on identifying the transcriptional and cell-signaling responses activated in A549 alveolar carcinoma cells incubated in the presence of A. fumigatus wild-type and ΔPrtT protease-deficient germinating conidia and culture filtrates (CF). Microarray analysis of exposed A549 cells identified distinct classes of genes whose expression is altered in the presence of germinating conidia and CF and suggested the involvement of both NFkB and MAPK signaling pathways in mediating the cellular response. Phosphoprotein analysis of A549 cells confirmed that JNK and ERK1/2 are phosphorylated in response to CF treatment in a protease-dependent manner. Inhibition of JNK or ERK1/2 kinase activity substantially decreased CF-induced cell damage, including cell peeling, actin-cytoskeleton damage, and reduction in metabolic activity and necrotic death. These results suggest that inhibition of MAPK-mediated host responses to treatment with A. fumigatus CF decreases cellular damage, a finding with possible clinical implications.
Overall design 3 independent controls (uninfected A549 cells) as ctrl2_1-3, 2 independent treatments of A549 cells with wild-type A. fumigatus culture filtrates (WT-CF2_1-2) and 2 independent treatments of A549 cells with wild-type A. fumigatus germinating conidia (WT-GC_2-3).
Contributor(s) Sharon H, Amar D, Shamir R, Osherov N
Citation(s) 21412410
Submission date Oct 28, 2010
Last update date Dec 06, 2018
Contact name Nir Osherov
Phone 972 3 640 9599
Fax 972 3 640 9160
Organization name Tel-Aviv University
Department Medical Microbiology and Immunology
Lab Aspergillus Lab Osherov
Street address Ramat Aviv Campus
City Tel-Aviv
ZIP/Postal code 69978
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (7)
GSM613611 WT-CF2_1
GSM613612 WT-CF2_2
GSM613613 Ctrl2_1
This SubSeries is part of SuperSeries:
GSE24986 Response of A549 cells treated with Aspergillus fumigatus
BioProject PRJNA133447

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24983_RAW.tar 14.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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