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Status |
Public on Jan 01, 2024 |
Title |
Bulk RNA-seq of Sox9-deficient intrahepatic biliary epithelial cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To define the regulatory role of Sox9 in intrahepatic biliary epithelium, we conducted bulk RNA-seq on biliary epithelial cells (BECs) from adult control (Sox9fl/fl) and conditional Sox9 knockout (Sox9cKO; Sox9fl/fl:Albumin-Cre) mice. Livers were dissociated to single cells and BECs isolated by FACS based on CD326 positivity. CD31+ and CD45+ cells were excluded by FACS. Gallbladder and extrahepatic tissues were excluded by dissection. RNA was isolated using Ambion RNAqueous Micro kit and libraries were made using Takara SMARTer Stranded Total RNA-seq Kit v3 Pico.
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Overall design |
Differential expression analysis of 3 Sox9cKO and 3 Control samples of BEC cells
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Contributor(s) |
Hrncir HR, Bombin S, Gracz AD |
Citation(s) |
38293117 |
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https://doi.org/10.1101/2024.01.15.574730
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Submission date |
Dec 05, 2023 |
Last update date |
Feb 13, 2024 |
Contact name |
Sergei Bombin |
E-mail(s) |
sbombin@augusta.edu
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Organization name |
Augusta University
|
Street address |
1120 15th St
|
City |
Augusta |
ZIP/Postal code |
30912 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA1048936 |