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Series GSE249385 Query DataSets for GSE249385
Status Public on Jan 01, 2024
Title Bulk RNA-seq of Sox9-deficient intrahepatic biliary epithelial cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To define the regulatory role of Sox9 in intrahepatic biliary epithelium, we conducted bulk RNA-seq on biliary epithelial cells (BECs) from adult control (Sox9fl/fl) and conditional Sox9 knockout (Sox9cKO; Sox9fl/fl:Albumin-Cre) mice. Livers were dissociated to single cells and BECs isolated by FACS based on CD326 positivity. CD31+ and CD45+ cells were excluded by FACS. Gallbladder and extrahepatic tissues were excluded by dissection. RNA was isolated using Ambion RNAqueous Micro kit and libraries were made using Takara SMARTer Stranded Total RNA-seq Kit v3 Pico.
Overall design Differential expression analysis of 3 Sox9cKO and 3 Control samples of BEC cells
Contributor(s) Hrncir HR, Bombin S, Gracz AD
Citation(s) 38293117
Submission date Dec 05, 2023
Last update date Feb 13, 2024
Contact name Adam Gracz
Organization name Emory University
Street address 201 Dowman Drive
City Atlanta
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM7943486 BEC_Control_1 [177]
GSM7943487 BEC_Sox9cKO_1 [178]
GSM7943488 BEC_Sox9cKO_2 [1770]
BioProject PRJNA1048936

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE249385_Normalized_counts.csv.gz 1.0 Mb (ftp)(http) CSV
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Raw data are available in SRA

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