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Series GSE249283 Query DataSets for GSE249283
Status Public on Feb 06, 2024
Title Bispecific dendritic-T Cell Engager potentiates anti-tumor immunity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Immune checkpoint inhibition treatment using aPD-1 monoclonal antibodies is a promising cancer immunotherapy approach. However, its effect on tumor immunity is narrow, as most patients do not respond to the treatment or suffer from recurrence. We show that the crosstalk between conventional type I dendritic cells (cDC1) and T cells is essential for an effective aPD-1-mediated anti-tumor response. Accordingly, we developed a Bispecific DC-T Cell Engager (BiCE), a reagent that facilitates physical interactions between PD-1+ T-cells and cDC1. BiCE treatment promoted the formation of active dendritic/T cell crosstalk in the tumor and tumor-draining lymph nodes. In vivo, single cell and physical interacting cell analysis demonstrated the distinct and superior immune reprogramming of the tumors and tumor-draining lymph nodes treated with BiCE, as compared to conventional aPD-1 treatment. BiCE introduces a new concept in immunotherapy, bridging of immune cells to potentiate cell circuits and communication pathways needed for effective anti-tumor immunity.
 
Overall design Dissecting the effect of BiCE on tumor immune microenvironment using single-cell RNA sequencing. Mice were inoculated with B16 cells and treated with either BiCE,aPD1/Synagis,aPD1 mAb mIg or untreated. Immune cells were harvested from tumors in early and late different time-points and underwent scRNAseq.
 
Contributor(s) Shapir-Itai Y, Barboy O, Salomon R, Bercovich A, Xie K, Winter E, Shami T, Porat Z, Neta E, Tanay A, Amit I, Dahan R
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Submission date Dec 04, 2023
Last update date Feb 07, 2024
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (187)
GSM7932884 AB13503
GSM7932885 AB13504
GSM7932886 AB13505
Relations
BioProject PRJNA1048445

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE249283_RAW.tar 104.9 Mb (http)(custom) TAR (of TXT)
GSE249283_metadata_dln_late_annotated.txt.gz 388.5 Kb (ftp)(http) TXT
GSE249283_metadata_q_SPID_BiSE.txt.gz 13.7 Kb (ftp)(http) TXT
GSE249283_metadata_tumor_early_HISAT_annotated.txt.gz 720.8 Kb (ftp)(http) TXT
GSE249283_metadata_tumor_late_annotated.txt.gz 415.2 Kb (ftp)(http) TXT
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Raw data are available in SRA

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