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| Status |
Public on Apr 24, 2024 |
| Title |
RNA-seq analysis of gene expression regulated by LSD1 in CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the RNA-seq analyses of in vitro activated and expanded CD8+ T cells, we found that GSK treatment significantly altered the transcriptome, with 241 genes upregulated and 426 genes downregulated respectively . Gene Ontology (GO) enrichment analysis revealed that the upregulated genes were highly enriched in biological processes related to cell killing and cytokine production.
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| Overall design |
Mouse CD8+ T cells were isolated from the spleens and lymph nodes of the mice using a biotin selection kitwith an EasySep™ Mouse CD8+ T Cell Isolation Kit according to the manufacturer’s manual. The CD8+ T cells were stimulated with 2 μg/ml plate-bound anti-CD3 (2μg/ml) and 2 μg/ml soluble anti-CD28 (2μg/ml) antibodies with the addition of 20 ng/mL hIL-2 at a density of 1 million/ml for 48 h. Activated CD8+ T cells were expanded with hIL2 in fresh medium for the indicated time before analysis. 0.5 μM GSK2879552 or vehicle control was added. Total RNA was extracted and used to poly(A)+ RNA enrichment by magnetic mRNA isolation kit for constructing library. The prepared libraries were sequenced at 150 bp from both ends.
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| Web link |
http://10.1038/s41467-024-48607-4
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| Contributor(s) |
Qiu F, Sheng W |
| Citation(s) |
38773088 |
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| Submission date |
Nov 29, 2023 |
| Last update date |
May 27, 2024 |
| Contact name |
Wanqiang Sheng |
| E-mail(s) |
nussheng@gmail.com
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| Organization name |
Zhejiang University School of Medicine
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| Department |
Department of Immunology
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| Lab |
The Sheng Lab
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| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1046395 |
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