GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE24876 Query DataSets for GSE24876
Status Public on Feb 04, 2011
Title Acute high dose X-irradiation-induced genomic changes in a lung tumor cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Genome variation profiling by genome tiling array
Summary Accidents with ionizing radiation (IR) often involve acute high dose exposures that can lead to acute radiation syndrome and late effects. IR can induce genomic lesions, cell death or carcinogenesis. Here, we investigated acute IR-induced cellular genomic signatures at the genome wide level. After exposing the adenocarcinoma cell line A549 to an acute 6 Gy 240 kV X-Ray dose, four surviving clonogenic cells were recovered by minimal dilution and further expanded and analyzed by cytogenetics, chromosome painting and tiling-path array CGH, with the non-irradiated clone0 serving as control. It was found that acute X-ray exposure induced changes in modal chromosome number and specific translocations in the four irradiation surviving clones. Furthermore, clone4 displayed an increased radiosensitivity at D > 5 Gy. Array CGH disclosed unique and recurrent genomic changes, predominantly gains, and disclosed fragile sites FRA3B and FRA16D as preferential regions of genomic alterations in all irradiated clones, which likely relates to irradiation-induced genomic stress. Gene expression analysis revealed a specific profile of 364 genes in clone4, of which p53 pathway genes may contribute to its increased radiosensitivity. IR-induced genomic changes AND fragile site expression highlight the capacity of a single acute radiation exposure to resculpture the genome of tumor cells by inflicting genomic stress.
Overall design Gene expression and array-CGH in A549 cell line was analysed after exposure to 6Gy X-rays at a dose rate of 1Gy/min.
Contributor(s) Muradyan A, Stabentheiner S, Klause S, Madle H, Meineke V, Ullmann R, Scherthan H
Citation(s) 21361782
Submission date Oct 21, 2010
Last update date Feb 22, 2018
Contact name Artur Muradyan
Organization name Max Planck Institute for Molecular Genetics
Street address Ihnestr. 73
City Berlin
ZIP/Postal code 14195
Country Germany
Platforms (2)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
GPL5114 MPIMG Homo sapiens 36k_arrayCGH8
Samples (13)
GSM611686 Clone0_sham_irradiated_rep1
GSM611687 Clone1_6Gy_rep1
GSM611688 Clone2_6Gy_rep1
BioProject PRJNA132019

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24876_RAW.tar 67.8 Mb (http)(custom) TAR (of GPR, TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap