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Series GSE248692 Query DataSets for GSE248692
Status Public on Jan 31, 2024
Title Gut bacteria-derived serotonin promotes immune tolerance in early life through regulation of neonatal gut T cells III
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The gut microbiota promotes immune system development in early life, but the interactions between the gut metabolome and immune cells in the neonatal gut remains largely undefined. Here, we demonstrate that the neonatal gut is uniquely enriched with neurotransmitters, including serotonin; specific gut bacteria produce serotonin directly while downregulating monoamine oxidase A to limit serotonin breakdown. Serotonin directly signals to T cells to increase intracellular indole-3-acetaldehdye to inhibit mTOR activation and thereby promotes the differentiation of regulatory T cells, both ex vivo and in vivo in the neonatal intestine. Oral gavage of serotonin into neonatal mice leads to long-term T cell-mediated antigen-specific immune tolerance towards both dietary antigens and commensal bacteria. Together, our study has uncovered an important role for unique gut bacteria to increase serotonin availability in the neonatal gut and a novel function of gut serotonin to shape T cell response to dietary antigens and commensal bacteria to promote immune tolerance in early life.
 
Overall design GF WT neonatal mice were given 50 μL of 5-HT (12.5 μg/mL) or vehicle (PBS) at P8 and P9 via oral gavage. The mice were then sacrificed at P14 and small intestine tissue was harvested. Total RNA was isolated and bulk RNA-seq performed using an Illumina NovaSeq 6000 sequencer. LEfSe followed by multiple hypotheses correction using signal-to-noise rate and false discovery rate analyses was used to discover genes with differential abundance. Differences at LEfSe p < 0.05 and FDR < 0.05 were considered significant.
 
Contributor(s) Sanidad KZ, Rager SL, Carrow HC, Ananthanarayanan A, Callaghan R, Hart LR, Li T, Ravisankar P, Brown JA, Amir M, Jin JC, Savage AR, Luo R, Rowdo FM, Martin ML, Silver RB, Guo C, Krumsiek J, Inohara N, Zeng MY
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Submission date Nov 27, 2023
Last update date Jan 31, 2024
Contact name Katherine Sanidad
E-mail(s) kzs4001@med.cornell.edu
Organization name Weill Cornell Medicine
Street address 413 E69th St
City New York
ZIP/Postal code 11365
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7918832 PBS_1
GSM7918833 PBS_2
GSM7918834 PBS_3
This SubSeries is part of SuperSeries:
GSE248694 Gut bacteria-derived serotonin promotes immune tolerance in early life through regulation of neonatal gut T cells
Relations
BioProject PRJNA1045603

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE248692_5HT_RNAseq_processed_S5.xlsx 48.2 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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