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Series GSE248554 Query DataSets for GSE248554
Status Public on Apr 02, 2024
Title Hepatocytes differentiate into intestinal epithelial cells through a hybrid epithelial/mesenchymal cell state in culture II
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We found that hepatocytes isolated from adult mouse livers can dedifferentiate into progenitor-like cells and exhibit a hybrid epithelial/mesenchymal phenotype in monolayer culture. dediHeps can re-differentiate into mature hepatocytes by forming aggregates and fetal intestinal progenitor cells (FIPCs) by forming organoids in three-dimensional culture. We conducted RNA-seq analyses to investigate the characteristincs of hepatocytes, dediHeps, dediHep aggregates, dediHep derived spherical organoids, dediHep derived budding organoids, FIPC derived spherical organoids, intestinal stem cell (ISC) derived budding organoids, and CLiP cells (Katsuda, 2016).
 
Overall design We performed RNA-seq analyses for hepatocytes, dediHeps, dediHep aggregates, dediHep-derived spherical organoids, dediHep-derived budding organoids, FIPC-derived spherical organoids, ISC-derived budding organoids, and CLiPs.
 
Contributor(s) Miura S, Horisawa K, Ohkawa Y, Suzuki A
Citation(s) 38750036
Submission date Nov 24, 2023
Last update date May 29, 2024
Contact name Atsushi Suzuki
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Division of Organogenesis and Regeneration
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (49)
GSM7916666 freshly isolated hepatocytes, replicate 1
GSM7916667 freshly isolated hepatocytes, replicate 2
GSM7916668 freshly isolated hepatocytes, replicate 3
Relations
BioProject PRJNA1044777

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE248554_UMI_count_matrix.txt.gz 1.2 Mb (ftp)(http) TXT
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Raw data are available in SRA

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