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Series GSE248442 Query DataSets for GSE248442
Status Public on Mar 01, 2024
Title Transcriptome analysis of Sodium-Glucose Cotransporter-2 Inhibitors in Acute Myocardial Infarction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Acute myocardial infarction (AMI) is the leading cause of mortality worldwide. Dapagliflozin (DAPA), a sodium-glucose cotransporter-2 inhibitor (SGLT2i), is a new type of oral antidiabetic agent. Here, we examined the effects of DAPA on AMI and investigated the potential mechanisms.Heart tissue specimens were collected from C57BL/6J mice induced by ligation of the left anterior descending coronary artery and treated with DAPA at 1 mg/kg/day dose for 4 weeks after surgery. Echocardiography, histological staining, and RNA sequencing (RNA-seq) of these specimens were performed. The differentially expressed genes of interest were confirmed by qualitative RT-PCR (RT-qPCR) and western blotting (WB). In vitro experiments were performed to evaluate the effect of DAPA on myosin light-chain 4 (Myl4) upregulation and reduction of autophagy following hypoxic treatment. As a result, DAPA could improve cardiac function post MI by upregulating Myl4 and reducing autophagy; this finding suggests that the molecular regulation associated with Myl4 might be an important mechanism of AMI treatment by SGLT2i.
 
Overall design RNA sequencing (RNA-Seq) of the cardiac tissues from C57BL/6J mice, which was induced by left anterior descending (LAD) coronary artery ligation, treated with DAPA at a dose of 1mg/kg/day for 4 weeks after surgery was conducted. Differentially expressed genes (DEGs) enriched in the mice heart after DAPA treatment were identified at the transcriptome level.
 
Contributor(s) Chen H
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Submission date Nov 22, 2023
Last update date Mar 01, 2024
Contact name Huiling Chen
E-mail(s) Chl1995@stu.cqmu.edu.cn
Phone +86 13308781767
Organization name Chongqing Medical University
Street address Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
City Chongqing
ZIP/Postal code 400016
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7914430 MI-1
GSM7914431 MI-2
GSM7914432 MI-3
Relations
BioProject PRJNA1044017

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE248442_DEG_combinded_nofiltered.xls.gz 4.2 Mb (ftp)(http) XLS
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Raw data are available in SRA

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