|
| Status |
Public on Apr 13, 2011 |
| Title |
Tet1 and hydroxymethylcytosine in transcription and DNA methylation fidelity (ChIP/DIP-Seq data) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
We analyzed the genome-wide binding of Tet1 in control (shScr) and Tet1 knockdown (shTet1) mouse ES cells using two different Tet1 antibodies (Tet1-C and Tet1-N). Furthermore, we generated genome-wide mapping of hydroxymethyl cytosine (hmC) and methyl cytosine (mC). We find that hmC, in contrast to mC, is also found at transcription start sites (TSSs), and that there is a significant overlap between Tet1 binding and hmC positive regions. Surprisingly, our results also suggest, that Tet1 has a role in transcriptional repression. We showed that Tet1 associates with Sin3A co-repressor complex, and by performing ChIP-sequencing of Sin3A, we find co-localisation of Tet1 and Sin3a throughout the genome
|
| |
|
| Overall design |
Examination of Tet1 and Sin3A binding as well as hmC and mC localization in mouse ES cells
|
| |
|
| Contributor(s) |
Williams K, Pedersen MT |
| Citation(s) |
21490601 |
| |
| Submission date |
Oct 20, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Kristine Williams |
| E-mail(s) |
kristine.williams@bric.dk
|
| Organization name |
University of Copenhagen
|
| Department |
BRIC
|
| Lab |
Helin
|
| Street address |
Ole Maaløes vej 5
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (17)
|
|
| This SubSeries is part of SuperSeries: |
| GSE24843 |
Tet1 and hydroxymethylcytosine in transcription and DNA methylation fidelity |
|
| Relations |
| BioProject |
PRJNA133431 |
| SRA |
SRP004033 |
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