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Series GSE248255 Query DataSets for GSE248255
Status Public on Jan 15, 2024
Title Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation (Figure4)
Organism Mus musculus
Experiment type Other
Summary Long-lived plasma cells (PCs) secrete antibodies that can provide sustained immunity against infection. It has been proposed that high affinity cells are preferentially selected into this compartment, potentiating the immune response. We used single cell RNA-seq to track the germinal center (GC) development of Ighg2A10 cells, specific for the Plasmodium falciparum circumsporozoite protein (PfCSP). Following immunization with Plasmodium sporozoites we identified 3 populations of cells in the GC light zone. One population expressed a gene signature associated with the initiation of PC differentiation and had an enhanced propensity to form PCs in vitro. Unexpectedly, the estimated affinity of this putative pre-PC population was indistinguishable from cells in the GC generally. This was also true when high- or low-avidity recombinant PfCSP proteins were used as immunogens. Immunization with low-avidity PfCSP did, however, induce increased affinity maturation. Collectively these findings suggest that the initiation of PC development in the GC occurs via an affinity independent process.
Overall design Ighg2A10 B cells were adoptively transferred into WT or MD4 mice which were immunized by CSP27 or CSP9. Spleens were collected on day14 post immunization and IgD- Ighg2A10 B cells were sorted and pooled for 10X Genomics single-cell RNA sequencing
Contributor(s) Gao X, Kelly HG, Cockburn IA
Citation(s) 38228150
Submission date Nov 20, 2023
Last update date Apr 15, 2024
Contact name Xin Gao
Phone 0450031169
Organization name The Australian National University
Department Immunology
Street address 131 Garran Rd
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM7910312 IgD- Ighg2A10, MD4_CSP9, VDJ
GSM7910313 IgD- Ighg2A10, MD4_CSP9, Transcriptome
GSM7910314 IgD- Ighg2A10, MD4_CSP9, Antibody_Capture
This SubSeries is part of SuperSeries:
GSE248377 Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation
BioProject PRJNA1043113

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Supplementary file Size Download File type/resource
GSE248255_Sample1_MD4_CSP9_Processed.tar.gz 102.6 Mb (ftp)(http) TAR
GSE248255_Sample2_MD4_CSP27_Processed.tar.gz 104.1 Mb (ftp)(http) TAR
GSE248255_Sample3_WT_CSP9_Processed.tar.gz 87.1 Mb (ftp)(http) TAR
GSE248255_Sample4_WT_CSP27_Processed.tar.gz 95.3 Mb (ftp)(http) TAR
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