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Series GSE248202 Query DataSets for GSE248202
Status Public on Jan 01, 2024
Title Lineage-specific intolerance to oncogenic drivers restricts histological transformation [scRNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Lung adenocarcinoma (LUAD) and small cell lung cancer (SCLC) are thought to originate from different epithelial cell types in the lung. Intriguingly, LUAD can histologically transform into SCLC following treatment with targeted therapies. Here we designed models to follow the conversion of LUAD to SCLC and found the barrier to histological transformation converges on tolerance to Myc, which we implicate as a lineage-specific driver of the pulmonary neuroendocrine cell. Histological transformations are frequently accompanied by activation of the Akt pathway. Manipulating this pathway permitted tolerance to Myc as an oncogenic driver, producing rare, stem-like cells, transcriptionally resembling the pulmonary basal lineage. These findings suggest histological transformation may require the plasticity inherent to the basal stem cell, enabling tolerance to previously incompatible oncogenic driver programs.
 
Overall design Single cell RNA sequencing was performed for 27 samples from genetically engineered mouse models. All samples were sorted from fresh, enzymatically dissociated lung tissues and, in most cases, triplicates were pooled before sequencing.
 
Contributor(s) Gardner EE, Earlie EM, Li K, Thomas J, Hubisz MJ, Stein BD, Zhang C, Cantley LC, Laughney AM, Varmus H
Citation(s) 38330136
Submission date Nov 19, 2023
Last update date Apr 02, 2024
Contact name Ashley M Laughney
E-mail(s) ashley.laughney@gmail.com
Organization name Weill Cornell Medicine
Department Physiology, Biophysics, and Systems Biology
Lab Laughney Lab
Street address 413 East 69th Street
City NEW YORK
State/province New York
ZIP/Postal code 10021
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (27)
GSM7908316 Lung, de novo SCLC, rep 1
GSM7908317 Lung, de novo SCLC, rep 2
GSM7908318 Lung, de novo SCLC, rep 3
This SubSeries is part of SuperSeries:
GSE248207 Lineage-specific intolerance to oncogenic drivers restricts histological transformation
Relations
BioProject PRJNA1042807

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE248202_Gardner_et-al-2023_processed_adata_TEC_merged_new_samples.h5ad.gz 20.8 Gb (ftp)(http) H5AD
GSE248202_Gardner_et-al-2023_processed_adata_additional_Pten_samples.h5ad.gz 225.7 Mb (ftp)(http) H5AD
GSE248202_Gardner_et-al-2023_processed_adata_atlas.h5ad.gz 14.9 Gb (ftp)(http) H5AD
GSE248202_Gardner_et-al-2023_processed_adata_histological_transformation_subset.h5ad.gz 2.2 Gb (ftp)(http) H5AD
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